The Involvement of Ethylene in Calcium-Induced Adventitious Root Formation in Cucumber Under Salt Stress

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Mar 3

PMID 30823363

Citations 15

Authors

Jian Yu

Lijuan Niu

Jihua Yu

Weibiao Liao

Jianming Xie

Jian Lv

Zhi Feng

Linli Hu

Mohammed Mujitaba Dawuda

Affiliations

Soon will be listed here.

Abstract

Calcium and ethylene are essential in plant growth and development. In this study, we investigated the effects of calcium and ethylene on adventitious root formation in cucumber explants under salt stress. The results revealed that 10 μM calcium chloride (CaCl₂) or 0.1 μM ethrel (ethylene donor) treatment have a maximum biological effect on promoting the adventitious rooting in cucumber under salt stress. Meanwhile, we investigated that removal of ethylene suppressed calcium ion (Ca)-induced the formation of adventitious root under salt stress indicated that ethylene participates in this process. Moreover, the application of Ca promoted the activities of 1-aminocyclopropane-l-carboxylic acid synthase (ACS) and ACC Oxidase (ACO), as well as the production of 1-aminocyclopropane-l-carboxylic acid (ACC) and ethylene under salt stress. Furthermore, we discovered that Ca greatly up-regulated the expression level of , and under salt stress. Meanwhile, Ca significantly down-regulated , , and but positively up-regulated the expression of and under salt stress; however, the application of Ca chelators or channel inhibitors could obviously reverse the effects of Ca on the expression of the above genes. These results indicated that Ca played a vital role in promoting the adventitious root development in cucumber under salt stress through regulating endogenous ethylene synthesis and activating the ethylene signal transduction pathway.

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