Quantitation of Rheumatoid Factors by Enzyme Immunoassay Using Biotinylated Human IgG

Overview

Journal J Immunol Methods

Publisher Elsevier

Specialties Allergy & Immunology
Pathology

Date 1986 Mar 13

PMID 3081654

Citations 5

Authors

M Zrein

G de Marcillac

M H Van Regenmortel

Affiliations

Soon will be listed here.

Abstract

An enzyme-linked immunosorbent assay (ELISA) using biotinylated human IgG for the detection of rheumatoid factor (RF) in human sera has been developed. This assay avoids some of the problems encountered with earlier ELISA methods used for RF detection. Accurate RF titers of human sera were determined using a simple computer program developed for curve fitting of ELISA data.

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