Biomass Accumulation and Cell Wall Structure of Rice Plants Overexpressing a Dirigent-Jacalin of Sugarcane () Under Varying Conditions of Water Availability

A sugarcane gene encoding a , , was induced under drought stress. To elucidate its biological function, we integrated a -overexpression construction into the rice Nipponbare genome via -mediated transformation. Two transgenic lines with a single copy gene in T were selected and evaluated in both the T and T generations. Transgenic lines had drastically improved survival rate under water deficit conditions, at rates close to 100%, while WT did not survive. Besides, transgenic lines had improved biomass production and higher tillering under water deficit conditions compared with WT plants. Reduced pectin and hemicellulose contents were observed in transgenic lines compared with wild-type plants under both well-watered and water deficit conditions, whereas cellulose content was unchanged in line #17 and reduced in line #29 under conditions of low water availability. Changes in lignin content under water deficit were only observed in line #17. However, improvements in saccharification were found in both transgenic lines along with changes in the expression of and secondary cell wall biosynthesis genes. -overexpression up-regulated the expression of the , , , and genes in rice stems under well-watered conditions. Taken together, our data suggest that has the potential for improving drought tolerance, plant biomass accumulation, and saccharification efficiency.

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References

Vijayan M, Chandra N . Lectins. Curr Opin Struct Biol. 1999; 9(6):707-14. DOI: 10.1016/s0959-440x(99)00034-2. View

Esen A, Blanchard D . A specific beta-glucosidase-aggregating factor is responsible for the beta-glucosidase null phenotype in maize. Plant Physiol. 2000; 122(2):563-72. PMC: 58893. DOI: 10.1104/pp.122.2.563. View

Tyagi , Mohanty . Rice transformation for crop improvement and functional genomics. Plant Sci. 2000; 158(1-2):1-18. DOI: 10.1016/s0168-9452(00)00325-3. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Williams C, Collier C, Nemacheck J, Liang C, Cambron S . A lectin-like wheat gene responds systemically to attempted feeding by avirulent first-instar Hessian fly larvae. J Chem Ecol. 2002; 28(7):1411-28. DOI: 10.1023/a:1016200619766. View

Chen L, Auh C, Chen F, Cheng X, Aljoe H, Dixon R . Lignin deposition and associated changes in anatomy, enzyme activity, gene expression, and ruminal degradability in stems of tall fescue at different developmental stages. J Agric Food Chem. 2002; 50(20):5558-65. DOI: 10.1021/jf020516x. View

Mason G, Provero P, Vaira A, Accotto G . Estimating the number of integrations in transformed plants by quantitative real-time PCR. BMC Biotechnol. 2002; 2:20. PMC: 137580. DOI: 10.1186/1472-6750-2-20. View

Shinozaki K, Yamaguchi-Shinozaki K, Seki M . Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol. 2003; 6(5):410-7. DOI: 10.1016/s1369-5266(03)00092-x. View

Ding J, Jia J, Yang L, Wen H, Zhang C, Liu W . Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes. J Agric Food Chem. 2004; 52(11):3372-7. DOI: 10.1021/jf049915d. View

10.

Wang X, Ma Q . Characterization of a jasmonate-regulated wheat protein related to a beta-glucosidase-aggregating factor. Plant Physiol Biochem. 2005; 43(2):185-92. DOI: 10.1016/j.plaphy.2005.01.018. View

11.

Thompson D . How do cell walls regulate plant growth?. J Exp Bot. 2005; 56(419):2275-85. DOI: 10.1093/jxb/eri247. View

12.

Jones D, Taylor W, Thornton J . The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci. 1992; 8(3):275-82. DOI: 10.1093/bioinformatics/8.3.275. View

13.

Jiang J, Han Y, Xing L, Xu Y, Xu Z, Chong K . Cloning and expression of a novel cDNA encoding a mannose-specific jacalin-related lectin from Oryza sativa. Toxicon. 2005; 47(1):133-9. DOI: 10.1016/j.toxicon.2005.10.010. View

14.

Verslues P, Agarwal M, Katiyar-Agarwal S, Zhu J, Zhu J . Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant J. 2006; 45(4):523-39. DOI: 10.1111/j.1365-313X.2005.02593.x. View

15.

Acevedo E, Hsiao T, Henderson D . Immediate and subsequent growth responses of maize leaves to changes in water status. Plant Physiol. 1971; 48(5):631-6. PMC: 396918. DOI: 10.1104/pp.48.5.631. View

16.

Hsiao T, OToole J, Yambao E, Turner N . Influence of Osmotic Adjustment on Leaf Rolling and Tissue Death in Rice (Oryza sativa L.). Plant Physiol. 1984; 75(2):338-41. PMC: 1066908. DOI: 10.1104/pp.75.2.338. View

17.

Toki S, Hara N, Ono K, Onodera H, Tagiri A, Oka S . Early infection of scutellum tissue with Agrobacterium allows high-speed transformation of rice. Plant J. 2006; 47(6):969-76. DOI: 10.1111/j.1365-313X.2006.02836.x. View

18.

Himmel M, Ding S, Johnson D, Adney W, Nimlos M, Brady J . Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science. 2007; 315(5813):804-7. DOI: 10.1126/science.1137016. View

19.

Xiao B, Huang Y, Tang N, Xiong L . Over-expression of a LEA gene in rice improves drought resistance under the field conditions. Theor Appl Genet. 2007; 115(1):35-46. DOI: 10.1007/s00122-007-0538-9. View

20.

Wasternack C . Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development. Ann Bot. 2007; 100(4):681-97. PMC: 2749622. DOI: 10.1093/aob/mcm079. View