Recent Paradigm Shift in the Assembly of Bacterial Tripartite Efflux Pumps and the Type I Secretion System

Overview

Journal J Microbiol

Specialty Microbiology

Date 2019 Feb 27

PMID 30806976

Citations 5

Authors

Inseong Jo

Jin-Sik Kim

Yongbin Xu

Jaekyung Hyun

Kangseok Lee

Nam-Chul Ha

Affiliations

Soon will be listed here.

Abstract

Tripartite efflux pumps and the type I secretion system of Gram-negative bacteria are large protein complexes that span the entire cell envelope. These complexes expel antibiotics and other toxic substances or transport protein toxins from bacterial cells. Elucidating the binary and ternary complex structures at an atomic resolution are crucial to understanding the assembly and working mechanism. Recent advances in cryoelectron microscopy along with the construction of chimeric proteins drastically shifted the assembly models. In this review, we describe the current assembly models from a historical perspective and emphasize the common assembly mechanism for the assembly of diverse tripartite pumps and type I secretion systems.

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