Global Transcriptome Analysis of Pig Induced Pluripotent Stem Cells Derived from Six and Four Reprogramming Factors

Overview

Journal Sci Data

Specialty Science

Date 2019 Feb 27

PMID 30806635

Citations 6

Authors

Tomokazu Fukuda

Koji Doi

Kenichiro Donai

Kouhei Takahashi

Hisato Kobayashi

Takashi Hirano

Katsuhiko Nishimori

Hiroshi Yasue

Affiliations

Soon will be listed here.

Abstract

Pigs are important, both for agriculture and as animal models for human diseases. However, due to the lack of embryonic stem cells, the possibility of genetic modification is quite limited. To overcome this limitation, induced pluripotent stem (iPS) cells have been derived from pigs. Despite the public availability of a large number of expression datasets from mice, rats, and primates-derived iPS cells, the expression profile of pig-derived iPS cells is quite limited. Furthermore, there is no dataset focused on the profiling of pig-derived iPS cell with six reprogramming factors (Oct3/4, Sox2, Klf4, c-Myc, Lin28, and Nanog). Here, we used Illumina RNA sequencing platform to characterize the mRNA expression of four-factor derived and six-factor derived pig iPS cells. We observed that the expression levels of whole genes in our established six factors derived iPS cells and parent fibroblast, and compared with that of iPS cells with four factors in public database. These data are valuable in understanding species difference in the reprogramming process of stem cells, and could help identify the key regulating genes involved in the process.

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