The Centriolar Adjunct⁻Appearance and Disassembly in Spermiogenesis and the Potential Impact on Fertility

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2019 Feb 23

PMID 30791486

Citations 21

Authors

Anastasiia S Garanina

Irina B Alieva

Elizaveta E Bragina

Emmanuelle Blanchard

Brigitte Arbeille

Fabrice Guerif

Svetlana Uzbekova

Rustem E Uzbekov

Affiliations

Soon will be listed here.

Abstract

During spermiogenesis, the proximal centriole forms a special microtubular structure: the centriolar adjunct. This structure appears at the spermatid stage, which is characterized by a condensed chromatin nucleus. We showed that the centriolar adjunct disappears completely in mature porcine spermatozoa. In humans, the centriolar adjunct remnants are present in a fraction of mature spermatids. For the first time, the structure of the centriolar adjunct in the cell, and its consequent impact on fertility, were examined. Ultrastructural analysis using transmission electron microscopy was performed on near 2000 spermatozoa per person, in two patients with idiopathic male sterility (IMS) and five healthy fertile donors. We measured the average length of the "proximal centriole + centriolar adjunct" complex in sections, where it had parallel orientation in the section plane, and found that it was significantly longer in the spermatozoa of IMS patients than in the spermatozoa of healthy donors. This difference was independent of chromatin condensation deficiency, which was also observed in the spermatozoa of IMS patients. We suggest that zygote arrest may be related to an incompletely disassembled centriolar adjunct in a mature spermatozoon. Therefore, centriolar adjunct length can be potentially used as a complementary criterion for the immaturity of spermatozoa in the diagnostics of IMS patients.

Citing Articles

Comment on: 'The proximal centriole age in spermatozoa is a potential reason for its different fate in the zygote after fertilization' Uzbekov and Avidor-Reiss 2024.

Uzbekov R, Avidor-Reiss T Open Biol. 2024; 14(3):230458.

PMID: 38442864 PMC: 10914506. DOI: 10.1098/rsob.230458.

CP110 and CEP135 Localize Near the Proximal Centriolar Remnants of Mice Spermatozoa.

Subbiah A, Caswell D, Turner K, Jaiswal A, Avidor-Reiss T MicroPubl Biol. 2024; 2024.

PMID: 38351906 PMC: 10862134. DOI: 10.17912/micropub.biology.001083.

The evolution of centriole degradation in mouse sperm.

Khanal S, Jaiswal A, Chowdanayaka R, Puente N, Turner K, Assefa K Nat Commun. 2024; 15(1):117.

PMID: 38168044 PMC: 10761967. DOI: 10.1038/s41467-023-44411-8.

The Endothelial Centrosome: Specific Features and Functional Significance for Endothelial Cell Activity and Barrier Maintenance.

Shakhov A, Churkina A, Kotlobay A, Alieva I Int J Mol Sci. 2023; 24(20).

PMID: 37895072 PMC: 10607758. DOI: 10.3390/ijms242015392.

Abnormal centriolar biomarker ratios correlate with unexplained bull artificial insemination subfertility: a pilot study.

Turner K, Achinger L, Kong D, Kluczynski D, Fishman E, Phillips A Sci Rep. 2023; 13(1):18338.

PMID: 37884598 PMC: 10603076. DOI: 10.1038/s41598-023-45162-8.

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