Antifungal Susceptibility of Species Isolated from Horticulturists with Onychomycosis in Piauí, Brazil

Overview

Journal Iran J Public Health

Date 2019 Feb 22

PMID 30788295

Citations 2

Authors

Mitra Mobin

Maria Walderez Szeszs

Juliana Possatto Takahashi

Marilena Martins

Daise Damaris Carnietto de Hippolito

Jhonatas Cley Santos Porto

Joao Batista Teles

Sidney Goncalo de Lima

Marcia de Souza Carvalho Melhem

Affiliations

Soon will be listed here.

Abstract

Background: We aimed to assess susceptibility pattern of species isolated from horticulturists with onychomycosis to four antifungal drugs and to compare the effectiveness of conventional identification methods with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS).

Methods: This study was conducted in a community garden located in Teresina, State of Piauí, Brazil, in the year 2014. The samples were identified through phenotypic methods and per MALDI-TOF MS, being used PCR as definitive identification test. The susceptibility pattern to four antifungal drugs was determined according to Clinical and Laboratory Standards Institute (CLSI).

Results: Fourteen clinical isolates from seven different species were identified by the phenotypic method and by MALDI-TOF MS, with an observed concordance of 71.4% between the two methods. (28.6%), (21.4%), and (both with 14.3%) were the most frequent species. With the exception of , all species were sensitive to the tested antifungal.

Conclusion: This is the first study of antifungal susceptibility of in Piauí, Brazil. With the exception of , no species showed resistance to the antifungal drugs used. This study suggests constants updates from the public databases used in MALDI-TOF MS to provide a rapid and accurate mycological diagnosis.

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