Transcriptome Sequencing Identifies Novel Immune Response Genes Highly Related to the Severity of Human Adenovirus Type 55 Infection

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Feb 22

PMID 30787914

Citations 10

Authors

Wen Xu

Zhe Xu

Lei Huang

En-Qiang Qin

Jie-Li Zhang

Peng Zhao

Bo Tu

Lei Shi

Wen-Gang Li

Wei-Wei Chen

Affiliations

Soon will be listed here.

Abstract

Human adenovirus type 55 (HAdV-55) is considered a highly virulent pathogen causing severe and even deadly pneumonia in immunocompetent people. The mechanisms of HAdV-55-induced initiation and progression of severe pneumonia remain ambiguous. In the current study, we endeavored to identify novel immune response genes which are substantially involved in the pathogenesis of severe inflammation in HAdV-55-infected patients. HAdV-55-infected patients with upper respiratory tract symptoms (minor patients) and pneumonia (severe patients) were enrolled. Through transcriptome sequencing and quantitative real-time PCR, the peripheral blood mononuclear cells of the patients were analyzed. We found that the expression of eight genes, including , , , , , , , and , were closely correlated with the severity of HAdV-55 infection. Most of these genes belong to interleukin-1 family or tumor necrosis factor (TNF) superfamily, respectively. The changes in gene expression were confirmed by Western blot assay. Our data will be crucial for deepening the understanding of the pathogenic mechanisms of severe pneumonia in HAdV-55 infection.

Citing Articles

Analysis of the Risk Factors for Plastic Bronchitis in Children with Severe Adenovirus Pneumonia: A Retrospective Study.

Xu X, Cai J, Fan H, Shi T, Yang D, Huang L Infect Drug Resist. 2024; 17:1011-1019.

PMID: 38505250 PMC: 10948331. DOI: 10.2147/IDR.S452347.

Initial Inoculum and the Severity of COVID-19: A Mathematical Modeling Study of the Dose-Response of SARS-CoV-2 Infections.

Fain B, Dobrovolny H Epidemiologia (Basel). 2022; 1(1):5-15.

PMID: 36417207 PMC: 9620883. DOI: 10.3390/epidemiologia1010003.

Randomized, double-blind, controlled trial of human anti-LIGHT monoclonal antibody in COVID-19 acute respiratory distress syndrome.

Perlin D, Neil G, Anderson C, Zafir-Lavie I, Raines S, Ware C J Clin Invest. 2021; 132(3).

PMID: 34871182 PMC: 8803341. DOI: 10.1172/JCI153173.

Plasma TNFSF13B and TNFSF14 Function as Inflammatory Indicators of Severe Adenovirus Pneumonia in Pediatric Patients.

Fan H, Lu B, Cao C, Li H, Yang D, Huang L Front Immunol. 2021; 11:614781.

PMID: 33542721 PMC: 7851050. DOI: 10.3389/fimmu.2020.614781.

Complete Genome Sequences of Two Human Adenovirus Type 55 Isolates from South Korea and the United States.

Hughes J, Yang Y, Fries A, Berry I, Pollio A, Fung C Microbiol Resour Announc. 2021; 10(5).

PMID: 33541877 PMC: 7862955. DOI: 10.1128/MRA.01347-20.

References

Kayagaki N, Yamaguchi N, Nakayama M, Takeda K, Akiba H, Tsutsui H . Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells. J Immunol. 1999; 163(4):1906-13. View

Chirmule N, Propert K, Magosin S, Qian Y, Qian R, Wilson J . Immune responses to adenovirus and adeno-associated virus in humans. Gene Ther. 1999; 6(9):1574-83. DOI: 10.1038/sj.gt.3300994. View

Fanger N, Maliszewski C, Schooley K, Griffith T . Human dendritic cells mediate cellular apoptosis via tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). J Exp Med. 1999; 190(8):1155-64. PMC: 2195665. DOI: 10.1084/jem.190.8.1155. View

Routes J, Ryan S, Clase A, Miura T, Kuhl A, POTTER T . Adenovirus E1A oncogene expression in tumor cells enhances killing by TNF-related apoptosis-inducing ligand (TRAIL). J Immunol. 2000; 165(8):4522-7. DOI: 10.4049/jimmunol.165.8.4522. View

Wang R, Zhang L, Zhang X, Moreno J, Luo X, Tondravi M . Differential regulation of the expression of CD95 ligand, receptor activator of nuclear factor-kappa B ligand (RANKL), TNF-related apoptosis-inducing ligand (TRAIL), and TNF-alpha during T cell activation. J Immunol. 2001; 166(3):1983-90. DOI: 10.4049/jimmunol.166.3.1983. View

Bossu P, Del Giudice E, Ciaramella A, Neumann D, Boraschi D . IL-18 and IL-18 receptors in the development of autoimmunity. Eur Cytokine Netw. 2001; 11(3):515-6. View

Migone T, Zhang J, Luo X, Zhuang L, Chen C, Hu B . TL1A is a TNF-like ligand for DR3 and TR6/DcR3 and functions as a T cell costimulator. Immunity. 2002; 16(3):479-92. DOI: 10.1016/s1074-7613(02)00283-2. View

Chew L, Pan H, Yu J, Tian S, Huang W, Zhang J . A novel secreted splice variant of vascular endothelial cell growth inhibitor. FASEB J. 2002; 16(7):742-4. DOI: 10.1096/fj.01-0757fje. View

Ehrlich S, Infante-Duarte C, Seeger B, Zipp F . Regulation of soluble and surface-bound TRAIL in human T cells, B cells, and monocytes. Cytokine. 2003; 24(6):244-53. DOI: 10.1016/s1043-4666(03)00094-2. View

10.

Kang Y, Kim W, Bae H, Kim D, Park Y, Park J . Involvement of TL1A and DR3 in induction of pro-inflammatory cytokines and matrix metalloproteinase-9 in atherogenesis. Cytokine. 2005; 29(5):229-35. DOI: 10.1016/j.cyto.2004.12.001. View

11.

Shayakhmetov D, Li Z, Ni S, Lieber A . Interference with the IL-1-signaling pathway improves the toxicity profile of systemically applied adenovirus vectors. J Immunol. 2005; 174(11):7310-9. DOI: 10.4049/jimmunol.174.11.7310. View

12.

Cotter M, Zaiss A, Muruve D . Neutrophils interact with adenovirus vectors via Fc receptors and complement receptor 1. J Virol. 2005; 79(23):14622-31. PMC: 1287577. DOI: 10.1128/JVI.79.23.14622-14631.2005. View

13.

Parr C, Gan C, Watkins G, Jiang W . Reduced vascular endothelial growth inhibitor (VEGI) expression is associated with poor prognosis in breast cancer patients. Angiogenesis. 2006; 9(2):73-81. DOI: 10.1007/s10456-006-9033-1. View

14.

Kawai T, Matsuyama T, Hosokawa Y, Makihira S, Seki M, Karimbux N . B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease. Am J Pathol. 2006; 169(3):987-98. PMC: 1698808. DOI: 10.2353/ajpath.2006.060180. View

15.

Hartman Z, Kiang A, Everett R, Serra D, Yang X, Clay T . Adenovirus infection triggers a rapid, MyD88-regulated transcriptome response critical to acute-phase and adaptive immune responses in vivo. J Virol. 2006; 81(4):1796-812. PMC: 1797572. DOI: 10.1128/JVI.01936-06. View

16.

Boraschi D, Dinarello C . IL-18 in autoimmunity: review. Eur Cytokine Netw. 2007; 17(4):224-52. View

17.

Pappu B, Borodovsky A, Zheng T, Yang X, Wu P, Dong X . TL1A-DR3 interaction regulates Th17 cell function and Th17-mediated autoimmune disease. J Exp Med. 2008; 205(5):1049-62. PMC: 2373838. DOI: 10.1084/jem.20071364. View

18.

Fang L, Adkins B, Deyev V, Podack E . Essential role of TNF receptor superfamily 25 (TNFRSF25) in the development of allergic lung inflammation. J Exp Med. 2008; 205(5):1037-48. PMC: 2373837. DOI: 10.1084/jem.20072528. View

19.

Barksby H, Nile C, Jaedicke K, Taylor J, Preshaw P . Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide. Clin Exp Immunol. 2009; 156(3):479-87. PMC: 2691977. DOI: 10.1111/j.1365-2249.2009.03920.x. View

20.

Falschlehner C, Schaefer U, Walczak H . Following TRAIL's path in the immune system. Immunology. 2009; 127(2):145-54. PMC: 2691779. DOI: 10.1111/j.1365-2567.2009.03058.x. View