In Vivo and In Vitro Antioxidant Activities of Methanol Extracts from Olive Leaves on

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2019 Feb 21

PMID 30781358

Citations 19

Authors

Siyuan Luo

Xuelian Jiang

Liping Jia

Chengyue Tan

Min Li

Qiuyu Yang

Yanlin Du

Chunbang Ding

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to evaluate the antioxidant activities of extracts from olive leaves (EOL). The main contents of EOL were determined by colorimetric methods. The antioxidant activities were assessed by measuring the scavenging free radicals in vitro. To investigate the antioxidant activity in vivo, we detected the survival of , under thermal stress. Subsequently the reactive oxygen species (ROS) level, activities of antioxidant enzymes, the expression of HSP-16.2 and the translocation of were measured. The results showed that, polyphenols was the main component. EOL could well scavenge DPPH and superoxide anion radicals in vitro. Compared to the control group, the survival rate of treated with EOL was extended by 10.43%, under heat stress. The ROS level was reduced, while the expression of was increased to protect the organism against the increasing ROS. The level of malondialdehyde (MDA) also decreased sharply. The activities of inner antioxidant enzymes, such as catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were potentiated, which might have had a correlation with the DAF-16 transcription factor that was induced-turned into the nuclear. Therefore, EOL showed a strong antioxidant ability in vitro and in vivo. Hence, it could be a potential candidate when it came to medicinal and edible plants.

Citing Articles

Brucine Entrapped Titanium Oxide Nanoparticle for Anticancer Treatment: An Study.

Almuqbil R Adv Pharmacol Pharm Sci. 2024; 2024:4646855.

PMID: 38529192 PMC: 10963080. DOI: 10.1155/2024/4646855.

Leaf extract of promotes anti-heat stress and antioxidant effects in .

Chuaijit S, Punsawad C, Winoto V, Plaingam W, Kongkaew I, Phetcharat A Front Pharmacol. 2024; 15:1331627.

PMID: 38515852 PMC: 10955098. DOI: 10.3389/fphar.2024.1331627.

Polysaccharides from with Great Antioxidant Ability and the Potential Application in Yogurt.

Wang Y, Zou Y, Fang Q, Feng R, Zhang J, Zhou W Molecules. 2024; 29(1).

PMID: 38202733 PMC: 10780003. DOI: 10.3390/molecules29010150.

Polyphenols in Inner Ear Neurobiology, Health and Disease: From Bench to Clinics.

Osakabe N, Modafferi S, Ontario M, Rampulla F, Zimbone V, Migliore M Medicina (Kaunas). 2023; 59(11).

PMID: 38004094 PMC: 10673256. DOI: 10.3390/medicina59112045.

Development of a Holistic In Vitro Cell-Free Approach to Determine the Redox Bioactivity of Agricultural Products.

Skaperda Z, Tekos F, Vardakas P, Nechalioti P, Kourti M, Patouna A Int J Mol Sci. 2023; 24(22).

PMID: 38003634 PMC: 10671064. DOI: 10.3390/ijms242216447.

References

Terashvili M, Pratt P, Gebremedhin D, Narayanan J, Harder D . Reactive oxygen species cerebral autoregulation in health and disease. Pediatr Clin North Am. 2006; 53(5):1029-37, xi. PMC: 2533262. DOI: 10.1016/j.pcl.2006.08.003. View

Owen R, Giacosa A, Hull W, Haubner R, Spiegelhalder B, Bartsch H . The antioxidant/anticancer potential of phenolic compounds isolated from olive oil. Eur J Cancer. 2000; 36(10):1235-47. DOI: 10.1016/s0959-8049(00)00103-9. View

Zhang Y, Mi D, Wang J, Luo Y, Yang X, Dong S . Constituent and effects of polysaccharides isolated from Sophora moorcroftiana seeds on lifespan, reproduction, stress resistance, and antimicrobial capacity in Caenorhabditis elegans. Chin J Nat Med. 2018; 16(4):252-260. DOI: 10.1016/S1875-5364(18)30055-4. View

Ahmed Mekheimer R, Sayed A, Ahmed E . Novel 1,2,4-triazolo[1,5-a]pyridines and their fused ring systems attenuate oxidative stress and prolong lifespan of Caenorhabiditis elegans. J Med Chem. 2012; 55(9):4169-77. DOI: 10.1021/jm2014315. View

Celino F, Yamaguchi S, Miura C, Ohta T, Tozawa Y, Iwai T . Tolerance of spermatogonia to oxidative stress is due to high levels of Zn and Cu/Zn superoxide dismutase. PLoS One. 2011; 6(2):e16938. PMC: 3041797. DOI: 10.1371/journal.pone.0016938. View

Wang B, Qu J, Luo S, Feng S, Li T, Yuan M . Optimization of Ultrasound-Assisted Extraction of Flavonoids from Olive () Leaves, and Evaluation of Their Antioxidant and Anticancer Activities. Molecules. 2018; 23(10). PMC: 6222376. DOI: 10.3390/molecules23102513. View

Ogg S, Paradis S, Gottlieb S, Patterson G, Lee L, Tissenbaum H . The Fork head transcription factor DAF-16 transduces insulin-like metabolic and longevity signals in C. elegans. Nature. 1997; 389(6654):994-9. DOI: 10.1038/40194. View

Shen P, Yue Y, Zheng J, Park Y . Caenorhabditis elegans: A Convenient In Vivo Model for Assessing the Impact of Food Bioactive Compounds on Obesity, Aging, and Alzheimer's Disease. Annu Rev Food Sci Technol. 2017; 9:1-22. DOI: 10.1146/annurev-food-030117-012709. View

Ishii N, Senoo-Matsuda N, Miyake K, Yasuda K, Ishii T, Hartman P . Coenzyme Q10 can prolong C. elegans lifespan by lowering oxidative stress. Mech Ageing Dev. 2004; 125(1):41-6. DOI: 10.1016/j.mad.2003.10.002. View

10.

Simon H, Levi-Schaffer F . Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis. 2001; 5(5):415-8. DOI: 10.1023/a:1009616228304. View

11.

Grintzalis K, Georgiou C, Schneider Y . An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination. Anal Biochem. 2015; 480:28-30. DOI: 10.1016/j.ab.2015.03.024. View

12.

Chattopadhyay D, Thirumurugan K . Longevity promoting efficacies of different plant extracts in lower model organisms. Mech Ageing Dev. 2018; 171:47-57. DOI: 10.1016/j.mad.2018.03.002. View

13.

Le Floch F, Tena M, Rios A, Valcarcel M . Supercritical fluid extraction of phenol compounds from olive leaves. Talanta. 2008; 46(5):1123-30. DOI: 10.1016/s0039-9140(97)00375-5. View

14.

Bokov A, Chaudhuri A, Richardson A . The role of oxidative damage and stress in aging. Mech Ageing Dev. 2004; 125(10-11):811-26. DOI: 10.1016/j.mad.2004.07.009. View

15.

Vayndorf E, Lee S, Liu R . Whole apple extracts increase lifespan, healthspan and resistance to stress in . J Funct Foods. 2013; 5(3):1236-1243. PMC: 3714114. DOI: 10.1016/j.jff.2013.04.006. View

16.

Harman D . The biologic clock: the mitochondria?. J Am Geriatr Soc. 1972; 20(4):145-7. DOI: 10.1111/j.1532-5415.1972.tb00787.x. View

17.

De Marino S, Festa C, Zollo F, Nini A, Antenucci L, Raimo G . Antioxidant activity and chemical components as potential anticancer agents in the olive leaf (Olea europaea L. cv Leccino.) decoction. Anticancer Agents Med Chem. 2014; 14(10):1376-85. DOI: 10.2174/1871520614666140804153936. View

18.

Bany I, Dong M, Koelle M . Genetic and cellular basis for acetylcholine inhibition of Caenorhabditis elegans egg-laying behavior. J Neurosci. 2003; 23(22):8060-9. PMC: 6740490. View

19.

Wang Z, Ma X, Li J, Cui X . Peptides from sesame cake extend healthspan of Caenorhabditis elegans via upregulation of skn-1 and inhibition of intracellular ROS levels. Exp Gerontol. 2016; 82:139-49. DOI: 10.1016/j.exger.2016.07.001. View

20.

Aruoma O . Free radicals, oxidative stress, and antioxidants in human health and disease. J Am Oil Chem Soc. 2020; 75(2):199-212. PMC: 7101596. DOI: 10.1007/s11746-998-0032-9. View