Novel Polyclonal Antibody-based Rapid Gold Sandwich Immunochromatographic Strip for Detecting the Major Royal Jelly Protein 1 (MRJP1) in Honey

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2019 Feb 20

PMID 30779780

Citations 1

Authors

Yifan Zhang

Yong Chen

Yiting Cai

Zongyan Cui

Jinjie Zhang

Xiaohou Wang

Lirong Shen

Affiliations

Soon will be listed here.

Abstract

Honey adulteration is becoming increasingly alarming incidents in food safety. Monitoring and detecting adulteration face greater challenges. Honey contains the major royal jelly proteins (MRJP) secreted by bee workers. To detect honey adulteration fast and accurately, a rapid gold sandwich immunochromatographic strip (GSIS) was developed based on two specific polyclonal antibodies (PoAbs) against the MRJP1, the most abundant protein of all MRJPs. We determined the best of pH value (pH 8.6) and PoAb SP-1 amount (5 μg/mL) in conjunction with colloidal. The cut-off value (sensitivity) of GSIS in detecting MRJP1 is 2.0 μg/mL in solution. Validation analysis with RJ, milk vetch honey, acacia honey and honey adulteration containing rice syrup and corn syrup with different ratios demonstrated that the GSIS could show consistent Test line (T line) when the test samples contain more than 30% pure honey or MRJP1 0.4 mg/g. The validation results by isotope ratio mass spectrometry on the same pure and all adulteration milk vetch honey samples showed the same information of GSIS test. The qualitative assay GSIS provided a valuable new way for honey authenticity and laid the foundation for the future application of GSIS with monoclonal antibodies in honey authentication.

Citing Articles

Physicochemical Characterization and Biological Properties of Pine Honey Produced across Greece.

Tsavea E, Vardaka F, Savvidaki E, Kellil A, Kanelis D, Bucekova M Foods. 2022; 11(7).

PMID: 35407030 PMC: 8997407. DOI: 10.3390/foods11070943.

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