Control of Proliferation and Differentiation in B Lymphocytes by Anti-Ig Antibodies and a Serum-derived Cofactor

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1978 May 1

PMID 307763

Citations 14

Authors

C L Sidman

E R UNANUE

Affiliations

Soon will be listed here.

Abstract

The effects of various anti-Ig antibodies on different B lymphocyte functions were investigated. With the proper accessory cofactor(s) derived from serum, anti-IgM antibodies induced a vigorous proliferative response in normal adult murine B cells, while polyspecific anti-Ig and anti-IgD had no effect. Without the required cofactor, all three anti-Ig antibodies were inhibitory for mitogenic responses. All three anti-Ig antibodies were also inhibitory for mitogen-induced antibody responses with or without the cofactor. Even with the required cofactor, neonatal B cells as well as adult C3H/HeJ B cells were not triggered into proliferation by anti-IgM. Finally, the cofactor required for anti-IgM-triggered mitogenesis was shown to be generated from serum by 2-mercaptoethanol and to be approximately 65,000 in molecular weight. These results indicate that, for at least some responses, B lymphocyte surface IgM molecules are involved in both triggering and suppression, depending both on the developmental state of the B cell and the presence or absence of accessory influences. In these experiments, IgD gave evidence only of being suppressive.

Citing Articles

Mechanism of augmentation of the antibody response in vitro by 2-mercaptoethanol in murine lymphocytes. I. 2-Mercaptoethanol-induced stimulation of the uptake of cystine, an essential amino acid.

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Dissociation of two signals required for activation of resting B cells.

Julius M, von Boehmer H, Sidman C Proc Natl Acad Sci U S A. 1982; 79(6):1989-93.

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Activation of murine B lymphocytes by anti-immunoglobulin is an inductive signal leading to immunoglobulin secretion.

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Induction of proliferation and differentiation of neoplastic B cells by anti-immunoglobulin and T-cell factors.

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Regulation of murine B cells through surface immunoglobulin. I. Monoclonal anti-delta antibody that induces allotype-specific proliferation.

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