Melittin Inhibition and Eradication Activity for Resistant Polymicrobial Biofilm Isolated from a Dairy Industry After Disinfection

Overview

Journal Int J Microbiol

Publisher Wiley

Specialty Microbiology

Date 2019 Feb 16

PMID 30766602

Citations 16

Authors

Emilia Galdiero

Antonietta Siciliano

Renato Gesuele

Valeria Di Onofrio

Annarita Falanga

Angela Maione

Renato Liguori

Giovanni Libralato

Marco Guida

Affiliations

Soon will be listed here.

Abstract

The emerging concern about the increase of antibiotic resistance and associated biofilm has encouraged scientists to look for alternative antibiotics such as antimicrobial peptides (AMPs). This study evaluated the ability of melittin to act as an antibacterial biofilm inhibitor and biofilm remover considering isolates from dairy industry. Minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), minimum biofilm inhibitory concentrations (MBICs), and biofilm removal activities were studied in polymicrobial biofilms produced from isolates. MIC and MBC were set at 1-3 g/mL and 25-50 g/mL for Gram-positive and Gram-negative bacteria, respectively. Results demonstrated a good MBIC reaching 85% inhibition ability and a good activity and better penetration in deeper layers against the mixed preformed biofilm, thereby increasing its activity against all isolates also at the lowest tested concentrations. Melittin showed interesting characteristics suggesting its potential to act as an antimicrobial agent for polymicrobial biofilm from dairy industry even in environmental isolates.

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