Overexpression of Apoptosis-related Protein, Survivin, in Fibroblasts from Patients with Systemic Sclerosis

Overview

Journal Ir J Med Sci

Specialty General Medicine

Date 2019 Feb 15

PMID 30761457

Citations 4

Authors

Mohammad Bagher Mahmoudi

Ehsan Farashahi Yazd

Farhad Gharibdoost

Mohammad Hasan Sheikhha

Elham Karimizadeh

Ahmadreza Jamshidi

Mahdi Mahmoudi

Affiliations

Soon will be listed here.

Abstract

Background/objectives: Recent studies suggest that, in addition to activation and hypersecretion of matrix components, fibroblasts from patients with systemic sclerosis (SSc) are resistant to apoptosis. Previous studies have shown that survivin, a member of inhibition of apoptosis (IAP) family, plays an important role in apoptosis resistance. Accordingly, we decided to study the expression of the most important members of IAP family in SSc fibroblasts, which can block apoptosis either by binding and inhibiting caspases or through caspase-independent mechanisms.

Method: Skin biopsy samples were obtained from 19 patients with diffuse cutaneous SSc (DcSSc) and 16 healthy controls. Dermal fibroblasts were cultured and the total RNA was isolated from cells followed by cDNA synthesis. Real-time PCR was performed using SYBR Green PCR master mix and specific primers for cIAP1, cIAP2, XIAP, and Survivin mRNA quantification.

Results: A significantly increased expression level of Survivin was observed in fibroblasts from SSc patients compared to controls (2.26-fold, P = 0.04). However, mRNA expression of cIAP1, cIAP2, and XIAP did not change significantly between cases and controls.

Conclusions: Our results showed that survivin is upregulated in SSc skin fibroblast which may lead to resistance to apoptosis. Further studies should be performed to reveal the role of survivin in apoptosis pathway of SSc fibroblasts.

Citing Articles

Human Cytomegalovirus and Human Herpesvirus 6 Coinfection of Dermal Fibroblasts Enhances the Pro-Inflammatory Pathway Predisposing to Fibrosis: The Possible Impact on Systemic Sclerosis.

Soffritti I, DAccolti M, Maccari C, Bini F, Mazziga E, De Conto F Microorganisms. 2022; 10(8).

PMID: 36014018 PMC: 9415275. DOI: 10.3390/microorganisms10081600.

The Role of Survivin and Transcription Factor FOXP1 in Scarring After Glaucoma Surgery.

Wang X, Huang Z, Zeng L, Jin X, Yan A, Zhang Y Transl Vis Sci Technol. 2022; 11(2):19.

PMID: 35142784 PMC: 8842717. DOI: 10.1167/tvst.11.2.19.

Modulation of microRNome by Human Cytomegalovirus and Human Herpesvirus 6 Infection in Human Dermal Fibroblasts: Possible Significance in the Induction of Fibrosis in Systemic Sclerosis.

Soffritti I, DAccolti M, Ravegnini G, Arcangeletti M, Maccari C, De Conto F Cells. 2021; 10(5).

PMID: 33946985 PMC: 8146000. DOI: 10.3390/cells10051060.

The contribution of mesenchymal transitions to the pathogenesis of systemic sclerosis.

Rosa I, Romano E, Fioretto B, Manetti M Eur J Rheumatol. 2020; 7(Suppl 3):S157-S164.

PMID: 31922472 PMC: 7647682. DOI: 10.5152/eurjrheum.2019.19081.

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