The Importance of Conserved Serine for C-Terminally Encoded Peptides Function Exertion in Apple

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Feb 15

PMID 30759748

Citations 5

Authors

Zipeng Yu

Yang Xu

Lin Liu

Yarong Guo

Xisen Yuan

Xinyu Man

Chang Liu

Guodong Yang

Jinguang Huang

Kang Yan

Chengchao Zheng

Changai Wu

Shizhong Zhang

Affiliations

Soon will be listed here.

Abstract

Background: The C-terminally encoded peptide (CEP) family has been shown to play vital roles in plant growth. Although a genome-wide analysis of this family has been performed in Arabidopsis, little is known regarding CEPs in apple ().

Methods: Here, a comprehensive bioinformatics approach was applied to identify MdCEPs in apple, and 12 genes were identified and distributed on 6 chromosomes.

Results: MdCEP1 peptide had an inhibitory effect on root growth of apple seedlings, indicating that MdCEP1 played a negative role in root development. In addition, the serine and glycine residues remained conserved within the CEP domains, and MdCEP1 lost its function after mutation of these two key amino acids, suggesting that Ser and Gly residues are crucial for MdCEPs-mediated root growth of apple. Encouragingly, multiple sequence alignment of 273 CEP domains showed that Ser residue was evolutionarily conserved in monocot and eudicot plants. MdCEP derivative (Ser to Cys) lost the ability to inhibit the root growth of , , , and L. and up-regulate the NO importer gene .

Conclusion: Taken together, Ser residue is crucial for CEP function exertion in higher land plants, at least in apple.

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