The Effect of Multispecies Probiotic Supplementation on Iron Status in Rats

Overview

Journal Biol Trace Elem Res

Date 2019 Feb 13

PMID 30746586

Citations 18

Authors

Katarzyna Skrypnik

Pawel Bogdanski

Marcin Schmidt

Joanna Suliburska

Affiliations

Soon will be listed here.

Abstract

A range of interactions between gut microbiota and iron (Fe) metabolism is described. Oral probiotics ameliorate host's iron status. However, this has been proven for single-strain probiotic supplements. Dose-dependence of beneficial probiotic supplementation effect on iron turnover remains unexplored. Our study aimed to investigate the effects of oral multispecies probiotic supplementation in two doses on iron status in rats. Thirty rats were randomized into three groups receiving multispecies probiotic supplement at a daily dose of 2.5 × 10 CFU (PA group, n = 10) and 1 × 10 CFU (PB group, n = 10) or placebo (KK group, n = 10). After 6 weeks, rats were sacrificed for analysis, blood samples, and organs (the liver, heart, kidneys, spleen, pancreas, femur, testicles, duodenum, and hair) were collected. The total fecal bacteria content was higher in the PB group vs. PA group. Unsaturated iron-binding capacity was higher in the PB group vs. KK group. Serum Fe was lower in both PA and PB vs. KK group. Iron content in the liver was higher in the PB group vs. KK group; in the pancreas, this was higher in the PB group vs. the KK and PA group, and in the duodenum, it was higher in both supplemented groups vs. the KK group. A range of alterations in zinc and copper status and correlations between analyzed parameters were found. Oral multispecies probiotic supplementation exerts dose-independent and beneficial effect on iron bioavailability and duodenal iron absorption in the rat model, induces a dose-independent iron shift from serum and intensifies dose-dependent pancreatic and liver iron uptake.

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References

Dinc G, Eser E, Saatli G, Cihan U, Oral A, Baydur H . The relationship between obesity and health related quality of life of women in a Turkish city with a high prevalence of obesity. Asia Pac J Clin Nutr. 2006; 15(4):508-15. View

Zhou Z, Johnson W, Kang Y . Regression of copper-deficient heart hypertrophy: reduction in the size of hypertrophic cardiomyocytes. J Nutr Biochem. 2008; 20(8):621-8. PMC: 3458645. DOI: 10.1016/j.jnutbio.2008.06.007. View

Al-Abdullah I, Ayala T, Panigrahi D, Kumar A, Kumar M . Neogenesis of pancreatic endocrine cells in copper-deprived rat models. Pancreas. 2000; 21(1):63-8. DOI: 10.1097/00006676-200007000-00053. View

Hoppe M, Onning G, Berggren A, Hulthen L . Probiotic strain Lactobacillus plantarum 299v increases iron absorption from an iron-supplemented fruit drink: a double-isotope cross-over single-blind study in women of reproductive age. Br J Nutr. 2015; 114(8):1195-202. PMC: 4594053. DOI: 10.1017/S000711451500241X. View

Skrypnik K, Bogdanski P, Loniewski I, Regula J, Suliburska J . Effect of probiotic supplementation on liver function and lipid status in rats. Acta Sci Pol Technol Aliment. 2018; 17(2):185-192. DOI: 10.17306/J.AFS.0554. View

Hoppe M, Onning G, Hulthen L . Freeze-dried Lactobacillus plantarum 299v increases iron absorption in young females-Double isotope sequential single-blind studies in menstruating women. PLoS One. 2017; 12(12):e0189141. PMC: 5728536. DOI: 10.1371/journal.pone.0189141. View

Aigner E, Strasser M, Haufe H, Sonnweber T, Hohla F, Stadlmayr A . A role for low hepatic copper concentrations in nonalcoholic Fatty liver disease. Am J Gastroenterol. 2010; 105(9):1978-85. DOI: 10.1038/ajg.2010.170. View

Tamaki M, Fujitani Y . [Role of zinc in type 2 diabetes]. Nihon Eiseigaku Zasshi. 2014; 69(1):15-23. DOI: 10.1265/jjh.69.15. View

Sanchez B, Delgado S, Blanco-Miguez A, Lourenco A, Gueimonde M, Margolles A . Probiotics, gut microbiota, and their influence on host health and disease. Mol Nutr Food Res. 2016; 61(1). DOI: 10.1002/mnfr.201600240. View

10.

Sondergaard L, Stoltenberg M, Doering P, Flyvbjerg A, Rungby J . Zinc ions in the endocrine and exocrine pancreas of zinc deficient rats. Histol Histopathol. 2006; 21(6):619-25. DOI: 10.14670/HH-21.619. View

11.

Nam H, Wang C, Zhang L, Zhang W, Hojyo S, Fukada T . ZIP14 and DMT1 in the liver, pancreas, and heart are differentially regulated by iron deficiency and overload: implications for tissue iron uptake in iron-related disorders. Haematologica. 2013; 98(7):1049-57. PMC: 3696608. DOI: 10.3324/haematol.2012.072314. View

12.

Bianconi E, Piovesan A, Facchin F, Beraudi A, Casadei R, Frabetti F . An estimation of the number of cells in the human body. Ann Hum Biol. 2013; 40(6):463-71. DOI: 10.3109/03014460.2013.807878. View

13.

Ng O . Iron, microbiota and colorectal cancer. Wien Med Wochenschr. 2016; 166(13-14):431-436. DOI: 10.1007/s10354-016-0508-4. View

14.

Coffey R, Nam H, Knutson M . Microarray analysis of rat pancreas reveals altered expression of Alox15 and regenerating islet-derived genes in response to iron deficiency and overload. PLoS One. 2014; 9(1):e86019. PMC: 3897611. DOI: 10.1371/journal.pone.0086019. View

15.

Yamanishi H, Iyama S, Yamaguchi Y, Kanakura Y, Iwatani Y . Total iron-binding capacity calculated from serum transferrin concentration or serum iron concentration and unsaturated iron-binding capacity. Clin Chem. 2003; 49(1):175-8. DOI: 10.1373/49.1.175. View

16.

Skrypnik K, Suliburska J . Association between the gut microbiota and mineral metabolism. J Sci Food Agric. 2017; 98(7):2449-2460. DOI: 10.1002/jsfa.8724. View

17.

Xiong X, Wang X, Li B, Chowdhury S, Lu Y, Srikant C . Pancreatic islet-specific overexpression of Reg3β protein induced the expression of pro-islet genes and protected the mice against streptozotocin-induced diabetes mellitus. Am J Physiol Endocrinol Metab. 2011; 300(4):E669-80. DOI: 10.1152/ajpendo.00600.2010. View

18.

Silva B, Faustino P . An overview of molecular basis of iron metabolism regulation and the associated pathologies. Biochim Biophys Acta. 2015; 1852(7):1347-59. DOI: 10.1016/j.bbadis.2015.03.011. View

19.

Fernandez-Real J, Lopez-Bermejo A, Ricart W . Cross-talk between iron metabolism and diabetes. Diabetes. 2002; 51(8):2348-54. DOI: 10.2337/diabetes.51.8.2348. View

20.

Suliburska J, Bogdanski P, Szulinska M . Iron excess disturbs metabolic status and relative gonad mass in rats on high fat, fructose, and salt diets. Biol Trace Elem Res. 2012; 151(2):263-8. PMC: 3548103. DOI: 10.1007/s12011-012-9548-9. View