Selection of DNA Cleavage Sites by Topoisomerase II Results from Enzyme-Induced Flexibility of DNA

Overview

Journal Cell Chem Biol

Publisher Cell Press

Specialty Biochemistry

Date 2019 Feb 5

PMID 30713098

Citations 10

Authors

Yunsu Jang

Heyjin Son

Sang-Wook Lee

Wonseok Hwang

Seung-Ryoung Jung

Jo Ann W Byl

Neil Osheroff

SangHwa Lee

Affiliations

Soon will be listed here.

Abstract

Topoisomerase II cleaves DNA at preferred sequences with different efficiencies; however, the mechanism of cleavage site selection is not known. Here we used single-molecule fluorescence assays that monitor several critical steps of DNA-topoisomerase II interactions, including binding/dissociation, bending/straightening, and cleavage/religation, and reveal that the cleavage site is selected mainly during the bending step. Furthermore, despite the sensitivity of the bending rate to the DNA sequence, it is not an intrinsic property of the DNA itself. Rather, it is determined by protein-DNA interactions.

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