Structural and Biomechanical Changes to Dentin Extracellular Matrix Following Chemical Removal of Proteoglycans

Overview

Journal Odontology

Specialty Dentistry

Date 2019 Feb 3

PMID 30710179

Citations 3

Authors

Ana Paula Farina

Cristina M P Vidal

Doglas Cecchin

Thaiane R Aguiar

Ana K Bedran-Russo

Affiliations

Soon will be listed here.

Abstract

Proteoglycans are biomacromolecules with significant biomineralization and structural roles in the dentin extracellular matrix. This study comprehensively assessed the mechanical properties and morphology of the dentin extracellular matrix following chemical removal of proteoglycans to elucidate the structural roles of proteoglycans in dentin. Dentin extracellular matrix was prepared from extracted teeth after complete tissue demineralization. Chemical removal of proteoglycans was carried-out using guanidine hydrochloride for up to 10 days. The removal of proteoglycans was determined by dimethylmethylene blue colorimetric assay and histological staining analyses using transmission electron microscopy and optical microscopy. The modulus of elasticity of dentin matrix was determined by a 3-point bending test method. Partial removal of proteoglycans induced significant modifications to the dentin matrix, particularly to type I collagen. Removal of proteoglycans significantly decreased the modulus of elasticity of dentin extracellular matrix (p < 0.0001). In conclusion, the subtle disruption of proteoglycans induces pronounced changes to the collagen network packing and the bulk modulus of elasticity of dentin matrix.

Citing Articles

Stability and remineralization of proteoglycan-infused dentin substrate.

Noschang R, Seebold D, Walter R, Rivera-Concepcion A, Alraheam I, Cardoso M Dent Mater. 2021; 37(11):1724-1733.

PMID: 34538503 PMC: 8578404. DOI: 10.1016/j.dental.2021.09.003.

On the bulk biomechanical behavior of densely cross-linked dentin matrix: The role of induced-glycation, regional dentin sites and chemical inhibitor.

Alania Y, Trevelin L, Hussain M, Zamperini C, Mustafa G, Bedran-Russo A J Mech Behav Biomed Mater. 2020; 103:103589.

PMID: 32090918 PMC: 7042333. DOI: 10.1016/j.jmbbm.2019.103589.

From Translation to Protein Degradation as Mechanisms for Regulating Biological Functions: A Review on the SLRP Family in Skeletal Tissues.

Zappia J, Joiret M, Sanchez C, Lambert C, Geris L, Muller M Biomolecules. 2020; 10(1).

PMID: 31947880 PMC: 7023458. DOI: 10.3390/biom10010080.

References

Barbosa I, Garcia S, Barbier-Chassefiere V, Caruelle J, Martelly I, Papy-Garcia D . Improved and simple micro assay for sulfated glycosaminoglycans quantification in biological extracts and its use in skin and muscle tissue studies. Glycobiology. 2003; 13(9):647-53. DOI: 10.1093/glycob/cwg082. View

Waddington R, Hall R, Embery G, Lloyd D . Changing profiles of proteoglycans in the transition of predentine to dentine. Matrix Biol. 2003; 22(2):153-61. DOI: 10.1016/s0945-053x(03)00019-2. View

Breschi L, Gobbi P, Lopes M, Prati C, Falconi M, Teti G . Immunocytochemical analysis of dentin: a double-labeling technique. J Biomed Mater Res A. 2003; 67(1):11-7. DOI: 10.1002/jbm.a.10048. View

Diaz Encarnacion M, Griffin M, Slezak J, Bergstralh E, Stegall M, Velosa J . Correlation of quantitative digital image analysis with the glomerular filtration rate in chronic allograft nephropathy. Am J Transplant. 2004; 4(2):248-56. DOI: 10.1046/j.1600-6143.2003.00311.x. View

Imbeni V, Kruzic J, Marshall G, Marshall S, Ritchie R . The dentin-enamel junction and the fracture of human teeth. Nat Mater. 2005; 4(3):229-32. DOI: 10.1038/nmat1323. View

Vogel K, Peters J . Histochemistry defines a proteoglycan-rich layer in bovine flexor tendon subjected to bending. J Musculoskelet Neuronal Interact. 2005; 5(1):64-9. View

Ho S, Sulyanto R, Marshall S, Marshall G . The cementum-dentin junction also contains glycosaminoglycans and collagen fibrils. J Struct Biol. 2005; 151(1):69-78. DOI: 10.1016/j.jsb.2005.05.003. View

Fisher M, Li Y, Seghatoleslami M, Dealy C, Kosher R . Heparan sulfate proteoglycans including syndecan-3 modulate BMP activity during limb cartilage differentiation. Matrix Biol. 2005; 25(1):27-39. DOI: 10.1016/j.matbio.2005.07.008. View

Provenzano P, Vanderby Jr R . Collagen fibril morphology and organization: implications for force transmission in ligament and tendon. Matrix Biol. 2005; 25(2):71-84. DOI: 10.1016/j.matbio.2005.09.005. View

10.

Goldberg M, Septier D, Oldberg A, Young M, Ameye L . Fibromodulin-deficient mice display impaired collagen fibrillogenesis in predentin as well as altered dentin mineralization and enamel formation. J Histochem Cytochem. 2005; 54(5):525-37. DOI: 10.1369/jhc.5A6650.2005. View

11.

Zhang G, Ezura Y, Chervoneva I, Robinson P, Beason D, Carine E . Decorin regulates assembly of collagen fibrils and acquisition of biomechanical properties during tendon development. J Cell Biochem. 2006; 98(6):1436-49. DOI: 10.1002/jcb.20776. View

12.

Dechichi P, Biffi J, Moura C, de Ameida A . A model of the early mineralization process of mantle dentin. Micron. 2006; 38(5):486-91. DOI: 10.1016/j.micron.2006.08.001. View

13.

Bedran-Russo A, Pereira P, Duarte W, Okuyama K, Yamauchi M . Removal of dentin matrix proteoglycans by trypsin digestion and its effect on dentin bonding. J Biomed Mater Res B Appl Biomater. 2007; 85(1):261-6. DOI: 10.1002/jbm.b.30944. View

14.

Mazzoni A, Pashley D, Ruggeri Jr A, Vita F, Falconi M, Di Lenarda R . Adhesion to chondroitinase ABC treated dentin. J Biomed Mater Res B Appl Biomater. 2007; 86(1):228-36. PMC: 3868986. DOI: 10.1002/jbm.b.31010. View

15.

Bedran-Russo A, Pashley D, Agee K, Drummond J, Miescke K . Changes in stiffness of demineralized dentin following application of collagen crosslinkers. J Biomed Mater Res B Appl Biomater. 2007; 86(2):330-4. DOI: 10.1002/jbm.b.31022. View

16.

Goldberg M, Ono M, Septier D, Bonnefoix M, Kilts T, Bi Y . Fibromodulin-deficient mice reveal dual functions for fibromodulin in regulating dental tissue and alveolar bone formation. Cells Tissues Organs. 2008; 189(1-4):198-202. PMC: 2756781. DOI: 10.1159/000151370. View

17.

Castellan C, Pereira P, Viana G, Chen S, Pauli G, Bedran-Russo A . Solubility study of phytochemical cross-linking agents on dentin stiffness. J Dent. 2010; 38(5):431-6. PMC: 2882861. DOI: 10.1016/j.jdent.2010.02.002. View

18.

Bedran-Russo A, Castellan C, Shinohara M, Hassan L, Antunes A . Characterization of biomodified dentin matrices for potential preventive and reparative therapies. Acta Biomater. 2010; 7(4):1735-41. PMC: 3050116. DOI: 10.1016/j.actbio.2010.12.013. View

19.

Bertassoni L, Stankoska K, Swain M . Insights into the structure and composition of the peritubular dentin organic matrix and the lamina limitans. Micron. 2011; 43(2-3):229-36. DOI: 10.1016/j.micron.2011.08.003. View

20.

Yamauchi N, Nagaoka H, Yamauchi S, Teixeira F, Miguez P, Yamauchi M . Immunohistological characterization of newly formed tissues after regenerative procedure in immature dog teeth. J Endod. 2011; 37(12):1636-41. DOI: 10.1016/j.joen.2011.08.025. View