Muscadine Grape Skin Extract Inhibits Prostate Cancer Cells by Inducing Cell-cycle Arrest, and Decreasing Migration Through Heat Shock Protein 40

Overview

Journal Heliyon

Specialty Social Sciences

Date 2019 Feb 2

PMID 30705983

Citations 6

Authors

Diane N Ignacio

Kimberly D Mason

Ezra C Hackett-Morton

Christopher Albanese

Lymor Ringer

William D Wagner

Paul C Wang

Michael A Carducci

Sushant K Kachhap

Channing J Paller

Janet Mendonca

Leo Li-Ying Chan

Bo Lin

Diane K Hartle

Jeffrey E Green

Collis A Brown

Tamaro S Hudson

Affiliations

Soon will be listed here.

Abstract

Previously we demonstrated that muscadine grape skin extract (MSKE), a natural product, significantly inhibited androgen-responsive prostate cancer cell growth by inducing apoptosis through the targeting of survival pathways. However, the therapeutic effect of MSKE on more aggressive androgen-independent prostate cancer remains unknown. This study examined the effects of MSKE treatment in metastatic prostate cancer using complementary PC-3 cells and xenograft model. MSKE significantly inhibited PC-3 human prostate cancer cell tumor growth and . The growth-inhibitory effect of MSKE appeared to be through the induction of cell-cycle arrest. This induction was accompanied by a reduction in the protein expression of Hsp40 and cell-cycle regulation proteins, cyclin D1 and NF-kBp65. In addition, MSKE induced p21 expression independent of wild-type p53 induced protein expression. Moreover, we demonstrate that MSKE significantly inhibited cell migration in PC-3 prostate cancer cells. Overall, these results demonstrate that MSKE inhibits prostate tumor growth and migration, and induces cell-cycle arrest by targeting Hsp40 and proteins involved in cell-cycle regulation and proliferation. This suggests that MSKE may also be explored either as a neo-adjuvant or therapeutic for castration resistant prostate cancer.

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