Trimester-specific Plasma Exosome MicroRNA Expression Profiles in Preeclampsia

Overview

Journal J Matern Fetal Neonatal Med

Publisher Informa Healthcare

Specialty Gynecology & Obstetrics

Date 2019 Feb 1

PMID 30700172

Citations 21

Authors

Eric Devor

Donna Santillan

Sabrina Scroggins

Akshaya Warrier

Mark Santillan

Affiliations

Soon will be listed here.

Abstract

To identify microRNAs (miRNAs) differentially expressed in plasma exosomes collected in women diagnosed with preeclampsia compared with women with uncomplicated pregnancies. Exosomes were purified from plasma samples obtained at each trimester from four women subsequently diagnosed with preeclampsia and from five matched healthy controls. RNA was purified from the exosomes, and expression of 368 miRNAs was profiled using A-Set TaqMan low density array (TLDA). One-third of the 368 miRNAs profiled are not expressed in exosomes. Further, those that are not expressed tend to be evolutionarily younger and have a significantly different mature sequence signature than do miRNAs that are expressed in exosomes. Among miRNAs that are expressed in exosomes, a total of eight (, , and ) were found to display statistically significant differential expression between women who developed preeclampsia as compared with those who did not. Moreover, half of these miRNAs (, , and ) displayed statistically significant differential expression in the first trimester. Not all miRNAs are expressed in exosomes. Those that tend to be evolutionarily older and have a significantly different mature sequence signature than those that are not. A few exosome-expressed miRNAs do display expression patterns in women subsequently diagnosed with preeclampsia that are significantly different than in women having an uncomplicated and, among these, several appear in the first trimester. These miRNAs are potential early markers of preeclampsia risk.

Citing Articles

Universal First-Trimester Screening Biomarkers for Diagnosis of Preeclampsia and Placenta Accreta Spectrum.

Timofeeva A, Fedorov I, Tarasova A, Sukhova Y, Kolodko V, Ivanets T Biomolecules. 2025; 15(2).

PMID: 40001531 PMC: 11852485. DOI: 10.3390/biom15020228.

EV-miRNA associated with environmental air pollution exposures in the MADRES cohort.

Foley H, Eckel S, Yang T, Vigil M, Chen X, Marsit C Environ Epigenet. 2024; 10(1):dvae019.

PMID: 39529802 PMC: 11552520. DOI: 10.1093/eep/dvae019.

Exosomal small RNA profiling in first-trimester maternal blood explores early molecular pathways of preterm preeclampsia.

Gal L, Fothi A, Orosz G, Nagy S, Than N, Orban T Front Immunol. 2024; 15:1321191.

PMID: 38455065 PMC: 10917917. DOI: 10.3389/fimmu.2024.1321191.

Early prediction of pre-eclampsia using circulating placental exosomes: Newer insights.

Rao A, Shinde U, Das D, Balasinor N, Madan T Indian J Med Res. 2023; 158(4):385-396.

PMID: 37987999 PMC: 10793818. DOI: 10.4103/ijmr.ijmr_2143_22.

The Long Journey of Extracellular Vesicles towards Global Scientific Acclamation.

Pirisinu M Adv Pharm Bull. 2023; 13(3):489-501.

PMID: 37646064 PMC: 10460810. DOI: 10.34172/apb.2023.049.

References

Yang Y, Han L, Yuan Y, Li J, Hei N, Liang H . Gene co-expression network analysis reveals common system-level properties of prognostic genes across cancer types. Nat Commun. 2014; 5:3231. PMC: 3951205. DOI: 10.1038/ncomms4231. View

Huang X, Yuan T, Tschannen M, Sun Z, Jacob H, Du M . Characterization of human plasma-derived exosomal RNAs by deep sequencing. BMC Genomics. 2013; 14:319. PMC: 3653748. DOI: 10.1186/1471-2164-14-319. View

Cai N, Wang Y, Zheng P . The microRNA-302-367 cluster suppresses the proliferation of cervical carcinoma cells through the novel target AKT1. RNA. 2012; 19(1):85-95. PMC: 3527729. DOI: 10.1261/rna.035295.112. View

Nardelli C, Iaffaldano L, Ferrigno M, Labruna G, Maruotti G, Quaglia F . Characterization and predicted role of the microRNA expression profile in amnion from obese pregnant women. Int J Obes (Lond). 2013; 38(3):466-9. DOI: 10.1038/ijo.2013.121. View

Pauli J, Repke J . Preeclampsia: Short-term and Long-term Implications. Obstet Gynecol Clin North Am. 2015; 42(2):299-313. DOI: 10.1016/j.ogc.2015.01.007. View

Weinstein J, Collisson E, Mills G, Mills Shaw K, Ozenberger B, Ellrott K . The Cancer Genome Atlas Pan-Cancer analysis project. Nat Genet. 2013; 45(10):1113-20. PMC: 3919969. DOI: 10.1038/ng.2764. View

Kandoth C, McLellan M, Vandin F, Ye K, Niu B, Lu C . Mutational landscape and significance across 12 major cancer types. Nature. 2013; 502(7471):333-339. PMC: 3927368. DOI: 10.1038/nature12634. View

Burton G, Yung H, Cindrova-Davies T, Charnock-Jones D . Placental endoplasmic reticulum stress and oxidative stress in the pathophysiology of unexplained intrauterine growth restriction and early onset preeclampsia. Placenta. 2008; 30 Suppl A:S43-8. PMC: 2684656. DOI: 10.1016/j.placenta.2008.11.003. View

Zhang Y, Diao Z, Su L, Sun H, Li R, Cui H . MicroRNA-155 contributes to preeclampsia by down-regulating CYR61. Am J Obstet Gynecol. 2010; 202(5):466.e1-7. DOI: 10.1016/j.ajog.2010.01.057. View

10.

Carty D, Delles C, Dominiczak A . Preeclampsia and future maternal health. J Hypertens. 2010; 28(7):1349-55. DOI: 10.1097/HJH.0b013e32833a39d0. View

11.

Ge J, Chen Z, Li R, Lu T, Xiao G . Upregulation of microRNA-196a and microRNA-196b cooperatively correlate with aggressive progression and unfavorable prognosis in patients with colorectal cancer. Cancer Cell Int. 2014; 14(1):128. PMC: 4269845. DOI: 10.1186/s12935-014-0128-2. View

12.

Yang S, Li H, Ge Q, Guo L, Chen F . Deregulated microRNA species in the plasma and placenta of patients with preeclampsia. Mol Med Rep. 2015; 12(1):527-34. DOI: 10.3892/mmr.2015.3414. View

13.

Wang Z, Banerjee S, Kong D, Li Y, Sarkar F . Down-regulation of Forkhead Box M1 transcription factor leads to the inhibition of invasion and angiogenesis of pancreatic cancer cells. Cancer Res. 2007; 67(17):8293-300. DOI: 10.1158/0008-5472.CAN-07-1265. View

14.

Li J, Wang Y, Luo J, Fu Z, Ying J, Yu Y . miR-134 inhibits epithelial to mesenchymal transition by targeting FOXM1 in non-small cell lung cancer cells. FEBS Lett. 2012; 586(20):3761-5. DOI: 10.1016/j.febslet.2012.09.016. View

15.

Campos C, Marques T, Pereira R, Sandrim V . Reduced circulating miR-196b levels is associated with preeclampsia. Pregnancy Hypertens. 2015; 4(1):11-3. DOI: 10.1016/j.preghy.2013.10.002. View

16.

Rossato M, Affandi A, Thordardottir S, Wichers C, Cossu M, Broen J . Association of MicroRNA-618 Expression With Altered Frequency and Activation of Plasmacytoid Dendritic Cells in Patients With Systemic Sclerosis. Arthritis Rheumatol. 2017; 69(9):1891-1902. DOI: 10.1002/art.40163. View

17.

Zhu X, Han T, Sargent I, Yin G, Yao Y . Differential expression profile of microRNAs in human placentas from preeclamptic pregnancies vs normal pregnancies. Am J Obstet Gynecol. 2009; 200(6):661.e1-7. DOI: 10.1016/j.ajog.2008.12.045. View

18.

Gilad S, Meiri E, Yogev Y, Benjamin S, Lebanony D, Yerushalmi N . Serum microRNAs are promising novel biomarkers. PLoS One. 2008; 3(9):e3148. PMC: 2519789. DOI: 10.1371/journal.pone.0003148. View

19.

Hamilton M, Rajapakshe K, Hartig S, Reva B, McLellan M, Kandoth C . Identification of a pan-cancer oncogenic microRNA superfamily anchored by a central core seed motif. Nat Commun. 2013; 4:2730. PMC: 3868236. DOI: 10.1038/ncomms3730. View

20.

Bartoszewski R, Brewer J, Rab A, Crossman D, Bartoszewska S, Kapoor N . The unfolded protein response (UPR)-activated transcription factor X-box-binding protein 1 (XBP1) induces microRNA-346 expression that targets the human antigen peptide transporter 1 (TAP1) mRNA and governs immune regulatory genes. J Biol Chem. 2011; 286(48):41862-41870. PMC: 3308892. DOI: 10.1074/jbc.M111.304956. View