Association of BAX Hypermethylation with Coronary Heart Disease is Specific to Individuals Aged over 70

Overview

Journal Medicine (Baltimore)

Specialty General Medicine

Date 2019 Jan 26

PMID 30681575

Citations 2

Authors

Limei Zhang

Huihui Ji

Yi Huang

Haochang Hu

Bin Li

Yong Yang

Hang Yu

Xiaoying Chen

Wenxia Li

Fang Liu

Shi Wang

Chunming Wang

Ke Chen

YingChun Bao

Haibo Liu

Shiwei Duan

Affiliations

Soon will be listed here.

Abstract

Introduction: As a member of B-cell lymphoma-2 (BCL-2) gene family, BCL-2 associated X (BAX) is important for cell apoptosis. In this work, we investigated the association of BAX promoter DNA methylation with coronary heart disease (CHD) in Han Chinese.

Methods: A SYBR green-based quantitative methylation specific PCR (qMSP) was used to test BAX methylation levels in 959 CHD cases and 514 controls.

Results: Although BAX methylation was not associated with CHD in the total samples, further breakdown analysis by age showed that BAX hypermethylation was significantly associated with CHD for individuals aged over 70 (median percentage of methylation ratio [PMR], 10.70% in cases versus (vs) 2.25% in controls, P =.046). Moreover, BAX methylation was associated with smoking and lipoprotein A (Lp(a)) for individuals aged over 70 (CHD: smoking P = .012, Lp(a) P = .001; non-CHD: smoking P = .051, Lp(a) P = .004). Further analysis of Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) data showed BAX expression was upregulated by 5-aza-2'-deoxycytidine demethylation agent (fold = 1.66, P = .038) and inversely correlated with BAX methylation (r = -0.428, P = 8E-05).

Conclusions: Our study supported that BAX hypermethylation might contribute to CHD risk via downregulation of BAX expression for individuals aged over 70.

Citing Articles

Epigenetic remodeling in heart failure with preserved ejection fraction.

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A male-specific association between AGTR1 hypermethylation and coronary heart disease.

Li X, Wu N, Ji H, Huang Y, Hu H, Li J Bosn J Basic Med Sci. 2019; 20(1):31-36.

PMID: 31538912 PMC: 7029202. DOI: 10.17305/bjbms.2019.4321.

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