Carbonic Anhydrase 6 Gene Variation Influences Oral Microbiota Composition and Caries Risk in Swedish Adolescents

Overview

Journal Sci Rep

Specialty Science

Date 2019 Jan 26

PMID 30679524

Citations 14

Authors

A Esberg

S Haworth

C Brunius

P Lif Holgerson

I Johansson

Affiliations

Soon will be listed here.

Abstract

Carbonic anhydrase VI (CA6) catalyses the reversible hydration of carbon dioxide in saliva with possible pH regulation, taste perception, and tooth formation effects. This study assessed effects of variation in the CA6 gene on oral microbiota and specifically the acidophilic and caries-associated Streptococcus mutans in 17-year old Swedish adolescents (n = 154). Associations with caries status and secreted CA6 protein were also evaluated. Single Nucleotide Polymorphisms (27 SNPs in 5 haploblocks) and saliva and tooth biofilm microbiota from Illumina MiSeq 16S rDNA (V3-V4) sequencing and culturing were analysed. Haploblock 4 (rs10864376, rs3737665, rs12138897) CCC associated with low prevalence of S. mutans (OR (95% CI): 0.5 (0.3, 0.8)), and caries (OR 0.6 (0.3, 0.9)), whereas haploblock 4 TTG associated with high prevalence of S. mutans (OR: 2.7 (1.2, 5.9)) and caries (OR: 2.3 (1.2, 4.4)). The TTG-haploblock 4 (represented by rs12138897(G)) was characterized by S. mutans, Scardovia wiggsiae, Treponema sp. HOT268, Tannerella sp. HOT286, Veillonella gp.1 compared with the CCC-haploblock 4 (represented by rs12138897(C)). Secreted CA6 in saliva was weakly linked to CA6 gene variation. In conclusion, the results indicate that CA6 gene polymorphisms influence S. mutans colonization, tooth biofilm microbiota composition and risk of dental caries in Swedish adolescents.

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References

Leinonen J, Kivela J, Parkkila S, Parkkila A, Rajaniemi H . Salivary carbonic anhydrase isoenzyme VI is located in the human enamel pellicle. Caries Res. 1999; 33(3):185-90. DOI: 10.1159/000016515. View

Kivela J, Parkkila S, Parkkila A, Leinonen J, Rajaniemi H . Salivary carbonic anhydrase isoenzyme VI. J Physiol. 1999; 520 Pt 2:315-20. PMC: 2269599. DOI: 10.1111/j.1469-7793.1999.t01-1-00315.x. View

Henkin R, Martin B, Agarwal R . Decreased parotid saliva gustin/carbonic anhydrase VI secretion: an enzyme disorder manifested by gustatory and olfactory dysfunction. Am J Med Sci. 2000; 318(6):380-91. DOI: 10.1097/00000441-199912000-00005. View

Bardow A, Moe D, Nyvad B, Nauntofte B . The buffer capacity and buffer systems of human whole saliva measured without loss of CO2. Arch Oral Biol. 2000; 45(1):1-12. DOI: 10.1016/s0003-9969(99)00119-3. View

Karhumaa P, Leinonen J, Parkkila S, Kaunisto K, Tapanainen J, Rajaniemi H . The identification of secreted carbonic anhydrase VI as a constitutive glycoprotein of human and rat milk. Proc Natl Acad Sci U S A. 2001; 98(20):11604-8. PMC: 58776. DOI: 10.1073/pnas.121172598. View

Johansson I, Hallmans G, Wikman A, Biessy C, Riboli E, Kaaks R . Validation and calibration of food-frequency questionnaire measurements in the Northern Sweden Health and Disease cohort. Public Health Nutr. 2002; 5(3):487-96. DOI: 10.1079/phn2001315. View

Gabriel S, Schaffner S, Nguyen H, Moore J, Roy J, Blumenstiel B . The structure of haplotype blocks in the human genome. Science. 2002; 296(5576):2225-9. DOI: 10.1126/science.1069424. View

Storm N, Van Den Boom D, Rodi C . MALDI-TOF mass spectrometry-based SNP genotyping. Methods Mol Biol. 2002; 212:241-62. DOI: 10.1385/1-59259-327-5:241. View

Vitorino R, Lobo M, Duarte J, Ferrer-Correia A, Tomer K, Dubin J . In vitro hydroxyapatite adsorbed salivary proteins. Biochem Biophys Res Commun. 2004; 320(2):342-6. DOI: 10.1016/j.bbrc.2004.05.169. View

10.

Kimoto M, Kishino M, Yura Y, Ogawa Y . A role of salivary carbonic anhydrase VI in dental plaque. Arch Oral Biol. 2005; 51(2):117-22. DOI: 10.1016/j.archoralbio.2005.04.007. View

11.

Uhlen M, Bjorling E, Agaton C, Szigyarto C, Amini B, Andersen E . A human protein atlas for normal and cancer tissues based on antibody proteomics. Mol Cell Proteomics. 2005; 4(12):1920-32. DOI: 10.1074/mcp.M500279-MCP200. View

12.

Supuran C . Carbonic anhydrases--an overview. Curr Pharm Des. 2008; 14(7):603-14. DOI: 10.2174/138161208783877884. View

13.

Gabriel S, Ziaugra L, Tabbaa D . SNP genotyping using the Sequenom MassARRAY iPLEX platform. Curr Protoc Hum Genet. 2009; Chapter 2:Unit 2.12. DOI: 10.1002/0471142905.hg0212s60. View

14.

Peres R, Camargo G, Mofatto L, Cortellazzi K, Santos M, Nobre-Dos-Santos M . Association of polymorphisms in the carbonic anhydrase 6 gene with salivary buffer capacity, dental plaque pH, and caries index in children aged 7-9 years. Pharmacogenomics J. 2009; 10(2):114-9. DOI: 10.1038/tpj.2009.37. View

15.

Chen T, Yu W, Izard J, Baranova O, Lakshmanan A, Dewhirst F . The Human Oral Microbiome Database: a web accessible resource for investigating oral microbe taxonomic and genomic information. Database (Oxford). 2010; 2010:baq013. PMC: 2911848. DOI: 10.1093/database/baq013. View

16.

Clarke G, Anderson C, Pettersson F, Cardon L, Morris A, Zondervan K . Basic statistical analysis in genetic case-control studies. Nat Protoc. 2011; 6(2):121-33. PMC: 3154648. DOI: 10.1038/nprot.2010.182. View

17.

Pertovaara M, Bootorabi F, Kuuslahti M, Pasternack A, Parkkila S . Novel carbonic anhydrase autoantibodies and renal manifestations in patients with primary Sjogren's syndrome. Rheumatology (Oxford). 2011; 50(8):1453-7. DOI: 10.1093/rheumatology/ker118. View

18.

Wu G, Chen J, Hoffmann C, Bittinger K, Chen Y, Keilbaugh S . Linking long-term dietary patterns with gut microbial enterotypes. Science. 2011; 334(6052):105-8. PMC: 3368382. DOI: 10.1126/science.1208344. View

19.

Yarat A, Ozturk L, Ulucan K, Akyuz S, Atala H, Isbir T . Carbonic anhydrase VI exon 2 genetic polymorphism in Turkish subjects with low caries experience (preliminary study). In Vivo. 2011; 25(6):941-4. View

20.

Genick U, Kutalik Z, Ledda M, Souza Destito M, Souza M, Cirillo C . Sensitivity of genome-wide-association signals to phenotyping strategy: the PROP-TAS2R38 taste association as a benchmark. PLoS One. 2011; 6(11):e27745. PMC: 3223210. DOI: 10.1371/journal.pone.0027745. View