Ascorbic Acid Encapsulated into Negatively Charged Liposomes Exhibits Increased Skin Permeation, Retention and Enhances Collagen Synthesis by Fibroblasts

Overview

Journal Sci Rep

Specialty Science

Date 2019 Jan 26

PMID 30679479

Citations 41

Authors

Lorena Maione-Silva

Elisandra Gava de Castro

Thais Leite Nascimento

Emilio Ramos Cintra

Larissa Cleres Moreira

Bertilha Alves Santana Cintra

Marize Campos Valadares

Eliana Martins Lima

Affiliations

Soon will be listed here.

Abstract

Ascorbic acid (AA) is widely used in cosmetic formulations due to its antioxidant property and ability to increase collagen synthesis. Here, we encapsulated AA in vesicles with different lipid compositions. Negative liposome charge favored AA skin retention, with accumulation of 37 ± 12 and 74 ± 23 μg/cm in the epidermis and dermis, respectively, after 6 hours. Drug flux was influenced by the formulation composition, and both the presence of cholesterol and the liposomes surface charge were able to increase the amount of AA crossing the skin. The formulation was stable for at least 30 days and promoted a 7-fold increase in flux compared to free AA. Additionally, liposomes were able to interact better with keratinocytes and fibroblasts membranes. In vitro efficacy studies demonstrated that associating AA to these liposomes resulted in increased effectiveness of type I collagen synthesis by fibroblasts and regeneration of UVA-induced damage in keratinocytes. Our results demonstrate the applicability of AA-negatively charged liposomes in promoting AA cutaneous permeation and increasing the retention and flux of this molecule in the skin. This formulation also increased AA stability and effectiveness, opening new perspectives for its application in view of reducing certain skin ageing outcomes.

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