Research Advances in Erythrocyte Regeneration Sources and Methods

Overview

Journal Cell Regen

Publisher Springer

Date 2019 Jan 24

PMID 30671230

Citations 8

Authors

Shuming Sun

Yuanliang Peng

Jing Liu

Affiliations

Soon will be listed here.

Abstract

Erythrocytes (red blood cells, RBCs) facilitate gas exchange in the lungs and transport oxygen to the tissues. The human body must maintain erythrocyte regeneration to support metabolically active cells and tissues. In many hematological diseases, erythrocyte regeneration is impaired. Researchers have studied erythrocyte regeneration for many years both and . In this review, we summarize the sources and main culture methods for generating mature and functional red blood cells . Hematopoietic stem cells (HSCs), embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are classic sources for erythrocyte regeneration. In addition, alternative sources such as immortalized adult human erythroid cell lines and transformed fibroblasts have also been generated and have produced functional red blood cells. The culture systems for erythrocytes differ among laboratories. Researchers hope that improvements in culture techniques may contribute to improved RBC outcomes for blood transfusions, drug delivery and the treatment of hematological diseases.

Citing Articles

Generating hematopoietic cells from human pluripotent stem cells: approaches, progress and challenges.

Zheng H, Chen Y, Luo Q, Zhang J, Huang M, Xu Y Cell Regen. 2023; 12(1):31.

PMID: 37656237 PMC: 10474004. DOI: 10.1186/s13619-023-00175-6.

Atomic Force Microscopy and High-Resolution Spectrophotometry for Study of Anoxemia and Normoxemia in Model Experiment In Vitro.

Kozlova E, Sherstyukova E, Sergunova V, Grechko A, Kuzovlev A, Lyapunova S Int J Mol Sci. 2023; 24(13).

PMID: 37446221 PMC: 10341442. DOI: 10.3390/ijms241311043.

Generation of Rh D-negative blood using CRISPR/Cas9.

Xu L, Zeng Q, Liang L, Yang Z, Qu M, Li H Cell Prolif. 2023; 56(11):e13486.

PMID: 37096780 PMC: 10623963. DOI: 10.1111/cpr.13486.

Mapping Human Pluripotent Stem Cell-derived Erythroid Differentiation by Single-cell Transcriptome Analysis.

Xin Z, Zhang W, Gong S, Zhu J, Li Y, Zhang Z Genomics Proteomics Bioinformatics. 2021; 19(3):358-376.

PMID: 34284135 PMC: 8864192. DOI: 10.1016/j.gpb.2021.03.009.

A new role of glutathione peroxidase 4 during human erythroblast enucleation.

Ouled-Haddou H, Messaoudi K, Demont Y, Lopes Dos Santos R, Carola C, Caulier A Blood Adv. 2020; 4(22):5666-5680.

PMID: 33211827 PMC: 7686909. DOI: 10.1182/bloodadvances.2020003100.

References

Kaufman D, Hanson E, LEWIS R, Auerbach R, Thomson J . Hematopoietic colony-forming cells derived from human embryonic stem cells. Proc Natl Acad Sci U S A. 2001; 98(19):10716-21. PMC: 58532. DOI: 10.1073/pnas.191362598. View

Neildez-Nguyen T, Wajcman H, Marden M, Bensidhoum M, Moncollin V, Giarratana M . Human erythroid cells produced ex vivo at large scale differentiate into red blood cells in vivo. Nat Biotechnol. 2002; 20(5):467-72. DOI: 10.1038/nbt0502-467. View

Migliaccio G, Di Pietro R, di Giacomo V, Di Baldassarre A, Migliaccio A, Maccioni L . In vitro mass production of human erythroid cells from the blood of normal donors and of thalassemic patients. Blood Cells Mol Dis. 2002; 28(2):169-80. DOI: 10.1006/bcmd.2002.0502. View

Uddin S, Ah-Kang J, Ulaszek J, Mahmud D, Wickrema A . Differentiation stage-specific activation of p38 mitogen-activated protein kinase isoforms in primary human erythroid cells. Proc Natl Acad Sci U S A. 2003; 101(1):147-52. PMC: 314153. DOI: 10.1073/pnas.0307075101. View

Giarratana M, Kobari L, Lapillonne H, Chalmers D, Kiger L, Cynober T . Ex vivo generation of fully mature human red blood cells from hematopoietic stem cells. Nat Biotechnol. 2004; 23(1):69-74. DOI: 10.1038/nbt1047. View

Krauss S, Lo A, Short S, Koury M, Mohandas N, Chasis J . Nuclear substructure reorganization during late-stage erythropoiesis is selective and does not involve caspase cleavage of major nuclear substructural proteins. Blood. 2005; 106(6):2200-5. PMC: 1895142. DOI: 10.1182/blood-2005-04-1357. View

Chang K, Nelson A, Cao H, Wang L, Nakamoto B, Ware C . Definitive-like erythroid cells derived from human embryonic stem cells coexpress high levels of embryonic and fetal globins with little or no adult globin. Blood. 2006; 108(5):1515-23. PMC: 1895504. DOI: 10.1182/blood-2005-11-011874. View

Miharada K, Hiroyama T, Sudo K, Nagasawa T, Nakamura Y . Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells. Nat Biotechnol. 2006; 24(10):1255-6. DOI: 10.1038/nbt1245. View

Qiu C, Olivier E, Velho M, Bouhassira E . Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells. Blood. 2007; 111(4):2400-8. PMC: 2234066. DOI: 10.1182/blood-2007-07-102087. View

10.

Palis J . Ontogeny of erythropoiesis. Curr Opin Hematol. 2008; 15(3):155-61. DOI: 10.1097/MOH.0b013e3282f97ae1. View

11.

Lu S, Feng Q, Park J, Vida L, Lee B, Strausbauch M . Biologic properties and enucleation of red blood cells from human embryonic stem cells. Blood. 2008; 112(12):4475-84. PMC: 2597123. DOI: 10.1182/blood-2008-05-157198. View

12.

Ma F, Ebihara Y, Umeda K, Sakai H, Hanada S, Zhang H . Generation of functional erythrocytes from human embryonic stem cell-derived definitive hematopoiesis. Proc Natl Acad Sci U S A. 2008; 105(35):13087-92. PMC: 2526552. DOI: 10.1073/pnas.0802220105. View

13.

Choi K, Yu J, Smuga-Otto K, Salvagiotto G, Rehrauer W, Vodyanik M . Hematopoietic and endothelial differentiation of human induced pluripotent stem cells. Stem Cells. 2009; 27(3):559-67. PMC: 2931800. DOI: 10.1634/stemcells.2008-0922. View

14.

BRETON-GORIUS J, Coulombel L, Guichard J, Teillet F, Vainchenker W . Association between leukemic erythroid progenitors and bone marrow macrophages. Blood Cells. 1991; 17(1):127-42; discussion 142-6. View

15.

Li M, Chen M, Han W, Fu X . How far are induced pluripotent stem cells from the clinic?. Ageing Res Rev. 2010; 9(3):257-64. DOI: 10.1016/j.arr.2010.03.001. View

16.

Lapillonne H, Kobari L, Mazurier C, Tropel P, Giarratana M, Zanella-Cleon I . Red blood cell generation from human induced pluripotent stem cells: perspectives for transfusion medicine. Haematologica. 2010; 95(10):1651-9. PMC: 2948089. DOI: 10.3324/haematol.2010.023556. View

17.

Sipp D . Challenges in the clinical application of induced pluripotent stem cells. Stem Cell Res Ther. 2010; 1(1):9. PMC: 3226303. DOI: 10.1186/scrt9. View

18.

Szabo E, Rampalli S, Risueno R, Schnerch A, Mitchell R, Fiebig-Comyn A . Direct conversion of human fibroblasts to multilineage blood progenitors. Nature. 2010; 468(7323):521-6. DOI: 10.1038/nature09591. View

19.

Chang C, Mitra K, Koya M, Velho M, Desprat R, Lenz J . Production of embryonic and fetal-like red blood cells from human induced pluripotent stem cells. PLoS One. 2011; 6(10):e25761. PMC: 3192723. DOI: 10.1371/journal.pone.0025761. View

20.

Keerthivasan G, Liu H, Gump J, Dowdy S, Wickrema A, Crispino J . A novel role for survivin in erythroblast enucleation. Haematologica. 2012; 97(10):1471-9. PMC: 3487547. DOI: 10.3324/haematol.2011.061093. View