Restricted Hematopoietic Progenitors and Erythropoiesis Require SCF from Leptin Receptor+ Niche Cells in the Bone Marrow

Overview

Journal Cell Stem Cell

Publisher Cell Press

Specialty Cell Biology

Date 2019 Jan 22

PMID 30661958

Citations 102

Authors

Stefano Comazzetto

Malea M Murphy

Stefano Berto

Elise Jeffery

Zhiyu Zhao

Sean J Morrison

Affiliations

Soon will be listed here.

Abstract

Hematopoietic stem cells (HSCs) are maintained in a perivascular niche in bone marrow, in which leptin receptor (LepR) stromal cells and endothelial cells synthesize factors required for HSC maintenance, including stem cell factor (SCF). An important question is why LepR cells are one hundred times more frequent than HSCs. Here, we show that SCF from LepR cells is also necessary to maintain many c-kit-restricted hematopoietic progenitors. Conditional deletion of Scf from LepR cells depleted common myeloid progenitors (CMPs), common lymphoid progenitors (CLPs), granulocyte-macrophage progenitors (GMPs), megakaryocyte-erythrocyte progenitors (MEPs), pre-megakaryocyte-erythrocyte progenitors (PreMegEs), and colony-forming units-erythroid (CFU-Es), as well as myeloid and erythroid blood cells. This was not caused by HSC depletion, as many other restricted progenitors were unaffected. Moreover, Scf deletion from endothelial cells depleted HSCs, but not progenitors. Early erythroid progenitors were closely associated with perisinusoidal LepR cells. This reveals cellular specialization within the niche: SCF from LepR cells is broadly required by HSCs and restricted progenitors while SCF from endothelial cells is required mainly by HSCs.

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