Effects of Low-Frequency Electromagnetic Field on Oxidative Stress in Selected Structures of the Central Nervous System

Overview

Journal Oxid Med Cell Longev

Publisher Wiley

Specialties Cell Biology
Endocrinology

Date 2019 Jan 17

PMID 30647806

Citations 3

Authors

Jan Budziosz

Agata Stanek

Aleksander Sieron

Joanna Witkos

Armand Cholewka

Karolina Sieron

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of the study was to evaluate the effects of a 28-day exposure to a 50 Hz electromagnetic field of 10 kVm on the oxidative stress in selected rat central nervous system (CNS) structures.

Material And Methods: Twenty male Wistar rats served as experimental subjects. Ten rats were exposed to an electromagnetic field with a frequency of 50 Hz, intensity of 10 kV/m, and magnetic induction of 4.3 pT for 22 hours a day. The control group of ten rats was subject to sham exposure. Homogenates of the frontal cortex, hippocampus, brainstem, hypothalamus, striatum, and cerebellum were evaluated for selected parameters of oxidative stress.

Results: Following the four-week exposure to a low-frequency electromagnetic field, the mean malondialdehyde levels and total oxidant status of CNS structures did not differ significantly between the experimental and control groups. However, the activities of antioxidant enzymes in brain structure homogenates were decreased except for frontal cortex catalase, glutathione peroxidase, and hippocampal glutathione reductase. The low-frequency electromagnetic field had no effect on the nonenzymatic antioxidant system of the examined brain structures except for the frontal cortex.

Conclusion: The four-week exposure of male rats to a low-frequency electromagnetic field did not affect oxidative stress in the investigated brain structures.

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References

Hardell L, Carlberg M . Mobile phones, cordless phones and the risk for brain tumours. Int J Oncol. 2009; 35(1):5-17. DOI: 10.3892/ijo_00000307. View

Paglia D, Valentine W . Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70(1):158-69. View

Habig W, Jakoby W . Assays for differentiation of glutathione S-transferases. Methods Enzymol. 1981; 77:398-405. DOI: 10.1016/s0076-6879(81)77053-8. View

Hardell L, Carlberg M, Hansson Mild K, Eriksson M . Case-control study on the use of mobile and cordless phones and the risk for malignant melanoma in the head and neck region. Pathophysiology. 2011; 18(4):325-33. DOI: 10.1016/j.pathophys.2011.06.001. View

Halliwell B . Oxidative stress and neurodegeneration: where are we now?. J Neurochem. 2006; 97(6):1634-58. DOI: 10.1111/j.1471-4159.2006.03907.x. View

Halliwell B . Antioxidant defence mechanisms: from the beginning to the end (of the beginning). Free Radic Res. 1999; 31(4):261-72. DOI: 10.1080/10715769900300841. View

Blesa J, Trigo-Damas I, Quiroga-Varela A, Jackson-Lewis V . Oxidative stress and Parkinson's disease. Front Neuroanat. 2015; 9:91. PMC: 4495335. DOI: 10.3389/fnana.2015.00091. View

Yakymenko I, Tsybulin O, Sidorik E, Henshel D, Kyrylenko O, Kyrylenko S . Oxidative mechanisms of biological activity of low-intensity radiofrequency radiation. Electromagn Biol Med. 2015; 35(2):186-202. DOI: 10.3109/15368378.2015.1043557. View

Lee B, Johng H, Lim J, Jeong J, Baik K, Nam T . Effects of extremely low frequency magnetic field on the antioxidant defense system in mouse brain: a chemiluminescence study. J Photochem Photobiol B. 2004; 73(1-2):43-8. DOI: 10.1016/j.jphotobiol.2003.10.003. View

10.

Stanek A, Cieslar G, Romuk E, Kasperczyk S, Sieron-Stoltny K, Birkner E . Decrease in antioxidant status of plasma and erythrocytes from patients with ankylosing spondylitis. Clin Biochem. 2010; 43(6):566-70. DOI: 10.1016/j.clinbiochem.2009.12.019. View

11.

Lupke M, Rollwitz J, Simko M . Cell activating capacity of 50 Hz magnetic fields to release reactive oxygen intermediates in human umbilical cord blood-derived monocytes and in Mono Mac 6 cells. Free Radic Res. 2004; 38(9):985-93. DOI: 10.1080/10715760400000968. View

12.

Wang H, Zhang X . Magnetic Fields and Reactive Oxygen Species. Int J Mol Sci. 2017; 18(10). PMC: 5666856. DOI: 10.3390/ijms18102175. View

13.

Halliwell B . Role of free radicals in the neurodegenerative diseases: therapeutic implications for antioxidant treatment. Drugs Aging. 2001; 18(9):685-716. DOI: 10.2165/00002512-200118090-00004. View

14.

Chater S, Abdelmelek H, Douki T, Garrel C, Favier A, Sakly M . Exposure to static magnetic field of pregnant rats induces hepatic GSH elevation but not oxidative DNA damage in liver and kidney. Arch Med Res. 2006; 37(8):941-6. DOI: 10.1016/j.arcmed.2006.05.010. View

15.

Johansson L, Borg L . A spectrophotometric method for determination of catalase activity in small tissue samples. Anal Biochem. 1988; 174(1):331-6. DOI: 10.1016/0003-2697(88)90554-4. View

16.

Oyanagui Y . Reevaluation of assay methods and establishment of kit for superoxide dismutase activity. Anal Biochem. 1984; 142(2):290-6. DOI: 10.1016/0003-2697(84)90467-6. View

17.

Poniedzialek B, Rzymski P, Karczewski J, Jaroszyk F, Wiktorowicz K . Reactive oxygen species (ROS) production in human peripheral blood neutrophils exposed in vitro to static magnetic field. Electromagn Biol Med. 2013; 32(4):560-8. DOI: 10.3109/15368378.2013.773910. View

18.

Jelenkovic A, Janac B, Pesic V, Jovanovic D, Vasiljevic I, Prolic Z . Effects of extremely low-frequency magnetic field in the brain of rats. Brain Res Bull. 2005; 68(5):355-60. DOI: 10.1016/j.brainresbull.2005.09.011. View

19.

Mahadik S, Mukherjee S . Free radical pathology and antioxidant defense in schizophrenia: a review. Schizophr Res. 1996; 19(1):1-17. DOI: 10.1016/0920-9964(95)00049-6. View

20.

Bonekamp N, Volkl A, Fahimi H, Schrader M . Reactive oxygen species and peroxisomes: struggling for balance. Biofactors. 2009; 35(4):346-55. DOI: 10.1002/biof.48. View