A Single Metabolite Production by BW25113 and Its Mutant Cultivated Under Microaerobic Conditions Using Glycerol or Glucose As a Carbon Source

Overview

Journal J Genet Eng Biotechnol

Specialty Biotechnology

Date 2019 Jan 17

PMID 30647652

Citations 2

Authors

Lolo Wal Marzan

Rinty Barua

Yasmin Akter

Md Arifuzzaman

Md Rafiqul Islam

Kazuyuki Shimizu

Affiliations

Soon will be listed here.

Abstract

Abundant, low prices and a highly reduced nature make glycerol to be an ideal feedstock for the production of reduced biochemicals and biofuels. has been paid much attention as the platform of microbial cell factories due to its high growth rate (giving higher metabolite production rate) and the capability of utilizing a wide range of carbon sources. However, one of the drawbacks of using as a platform is its mixed metabolite formation under anaerobic conditions. In the present study, it was shown that ethanol could be exclusively produced from glycerol by the wild type , while d-lactic acid could be exclusively produced from glucose by mutant, where the glucose uptake rate could be increased by this mutant as compared to the wild type strain. It was also shown that the growth rate is significantly reduced in mutant for the case of using glycerol as a carbon source due to redox imbalance. The metabolic regulation mechanisms behind the fermentation characteristic were clarified to some extent.

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References

Paulo da Silva G, Mack M, Contiero J . Glycerol: a promising and abundant carbon source for industrial microbiology. Biotechnol Adv. 2008; 27(1):30-9. DOI: 10.1016/j.biotechadv.2008.07.006. View

Dessein A, Schwartz M, Ullmann A . Catabolite repression in Escherichia coli mutants lacking cyclic AMP. Mol Gen Genet. 1978; 162(1):83-7. DOI: 10.1007/BF00333853. View

Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M . Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. 2006; 2:2006.0008. PMC: 1681482. DOI: 10.1038/msb4100050. View

Zhu J, Shimizu K . The effect of pfl gene knockout on the metabolism for optically pure D-lactate production by Escherichia coli. Appl Microbiol Biotechnol. 2003; 64(3):367-75. DOI: 10.1007/s00253-003-1499-9. View

Pagliaro M, Ciriminna R, Kimura H, Rossi M, Della Pina C . From glycerol to value-added products. Angew Chem Int Ed Engl. 2007; 46(24):4434-40. DOI: 10.1002/anie.200604694. View

Munjal N, Mattam A, Pramanik D, Srivastava P, Yazdani S . Modulation of endogenous pathways enhances bioethanol yield and productivity in Escherichia coli. Microb Cell Fact. 2012; 11:145. PMC: 3539902. DOI: 10.1186/1475-2859-11-145. View

Kochanowski K, Volkmer B, Gerosa L, Haverkorn van Rijsewijk B, Schmidt A, Heinemann M . Functioning of a metabolic flux sensor in Escherichia coli. Proc Natl Acad Sci U S A. 2013; 110(3):1130-5. PMC: 3549114. DOI: 10.1073/pnas.1202582110. View

Datsenko K, Wanner B . One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A. 2000; 97(12):6640-5. PMC: 18686. DOI: 10.1073/pnas.120163297. View

Schubert C . Can biofuels finally take center stage?. Nat Biotechnol. 2006; 24(7):777-84. DOI: 10.1038/nbt0706-777. View

10.

Doi Y, Ikegami Y . Pyruvate formate-lyase is essential for fumarate-independent anaerobic glycerol utilization in the Enterococcus faecalis strain W11. J Bacteriol. 2014; 196(13):2472-80. PMC: 4054171. DOI: 10.1128/JB.01512-14. View

11.

Dien B, Cotta M, Jeffries T . Bacteria engineered for fuel ethanol production: current status. Appl Microbiol Biotechnol. 2003; 63(3):258-66. DOI: 10.1007/s00253-003-1444-y. View

12.

Hansen A, Zhang Q, Lyne P . Ethanol-diesel fuel blends -- a review. Bioresour Technol. 2004; 96(3):277-85. DOI: 10.1016/j.biortech.2004.04.007. View

13.

Perrenoud A, Sauer U . Impact of global transcriptional regulation by ArcA, ArcB, Cra, Crp, Cya, Fnr, and Mlc on glucose catabolism in Escherichia coli. J Bacteriol. 2005; 187(9):3171-9. PMC: 1082841. DOI: 10.1128/JB.187.9.3171-3179.2005. View

14.

Durnin G, Clomburg J, Yeates Z, Alvarez P, Zygourakis K, Campbell P . Understanding and harnessing the microaerobic metabolism of glycerol in Escherichia coli. Biotechnol Bioeng. 2009; 103(1):148-61. DOI: 10.1002/bit.22246. View

15.

Murarka A, Dharmadi Y, Yazdani S, Gonzalez R . Fermentative utilization of glycerol by Escherichia coli and its implications for the production of fuels and chemicals. Appl Environ Microbiol. 2007; 74(4):1124-35. PMC: 2258577. DOI: 10.1128/AEM.02192-07. View

16.

Dharmadi Y, Murarka A, Gonzalez R . Anaerobic fermentation of glycerol by Escherichia coli: a new platform for metabolic engineering. Biotechnol Bioeng. 2006; 94(5):821-9. DOI: 10.1002/bit.21025. View

17.

Lin Y, Tanaka S . Ethanol fermentation from biomass resources: current state and prospects. Appl Microbiol Biotechnol. 2005; 69(6):627-42. DOI: 10.1007/s00253-005-0229-x. View

18.

Ohta K, Beall D, Mejia J, Shanmugam K, Ingram L . Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. Appl Environ Microbiol. 1991; 57(4):893-900. PMC: 182819. DOI: 10.1128/aem.57.4.893-900.1991. View

19.

Yazdani S, Gonzalez R . Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. Curr Opin Biotechnol. 2007; 18(3):213-9. DOI: 10.1016/j.copbio.2007.05.002. View

20.

Zhu J, Shimizu K . Effect of a single-gene knockout on the metabolic regulation in Escherichia coli for D-lactate production under microaerobic condition. Metab Eng. 2005; 7(2):104-15. DOI: 10.1016/j.ymben.2004.10.004. View