Characterization of Furnace Oil Bioremediation Potential of Hydrocarbonoclastic Bacteria Isolated from Petroleum Contaminated Sites of the Sundarbans, Bangladesh

Overview

Journal J Genet Eng Biotechnol

Specialty Biotechnology

Date 2019 Jan 17

PMID 30647647

Authors

Lolo Wal Marzan

Tasrin Sultana

Md Mahbub Hasan

Sohana Akter Mina

Md Rafiqul Islam

A G M Rakibuzzaman

Md Iqbal Hassan Khan

Affiliations

Soon will be listed here.

Abstract

Spillage of furnace oil is a more frequent event in recent times. In this study, environmental samples from furnace oil spillage sites of the Shela River, the Sundarbans, Bangladesh, were collected after three weeks of spillage. Serial dilution was applied and total seven bacterial isolates were separated as pure cultures. The oil-degrading potentiality of all seven isolates was further assessed, confirmed and compared with the growth pattern in furnace oil supplemented media, 2, 6-dichlorophenolindophenol test, and gravimetric analysis. After 7 days of incubation, isolates SS, RW, and SB degraded 56%, 43%, and 52% of supplemented furnace oil, respectively. The top three hydrocarbonoclastic bacterial isolates were selected as potential and identified as (SS), sp. (RW), and sp. (SB). All three isolates showed significant oil-degrading capacity compared to negative control, when incubated in sterile pond water supplemented with 2% furnace oil, suggesting them as potential bioremediation agents.

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