Gelatin Based Polymer Cell Coating Improves Bone Marrow-Derived Cell Retention in the Heart After Myocardial Infarction

Overview

Journal Stem Cell Rev Rep

Publisher Springer

Specialty Cell Biology

Date 2019 Jan 16

PMID 30644039

Citations 13

Authors

Anuhya Gottipati

Lakshman Chelvarajan

Hsuan Peng

Raymond Kong

Calvin F Cahall

Cong Li

Himi Tripathi

Ahmed Al-Darraji

Shaojing Ye

Eman Elsawalhy

Ahmed Abdel-Latif

Brad J Berron

Affiliations

Soon will be listed here.

Abstract

Background: Acute myocardial infarction (AMI) and the ensuing ischemic heart disease are approaching an epidemic state. Limited stem cell retention following intracoronary administration has reduced the clinical efficacy of this novel therapy. Polymer based cell coating is biocompatible and has been shown to be safe. Here, we assessed the therapeutic utility of gelatin-based biodegradable cell coatings on bone marrow derived cell retention in ischemic heart.

Methods: Gelatin based cell coatings were formed from the surface-mediated photopolymerization of 3% gelatin methacrylamide and 1% PEG diacrylate. Cell coating was confirmed using a multimodality approach including flow cytometry, imaging flow cytometry (ImageStream System) and immunohistochemistry. Biocompatibility of cell coating, metabolic activity of coated cells, and the effect of cell coating on the susceptibility of cells for engulfment were assessed using in vitro models. Following myocardial infarction and GFP+ BM-derived mesenchymal stem cell transplantation, flow cytometric and immunohistochemical assessment of retained cells was performed.

Results: Coated cells are viable and metabolically active with coating degrading within 72 h in vitro. Importantly, cell coating does not predispose bone marrow cells to aggregation or increase their susceptibility to phagocytosis. In vitro and in vivo studies demonstrated no evidence of heightened immune response or increased phagocytosis of coated cells. Cell transplantation studies following myocardial infarction proved the improved retention of coated bone marrow cells compared to uncoated cells.

Conclusion: Gelation based polymer cell coating is biologically safe and biodegradable. Therapies employing these strategies may represent an attractive target for improving outcomes of cardiac regenerative therapies in human studies.

Citing Articles

Identification and validation of key genes associated with cell senescence in acute myocardial infarction.

Zhao W, Zhu G, Chu T, Wu L, Li H, Zhen Q Front Cardiovasc Med. 2025; 12:1499157.

PMID: 40046960 PMC: 11880263. DOI: 10.3389/fcvm.2025.1499157.

Hydrogel-based cardiac repair and regeneration function in the treatment of myocardial infarction.

Xu Q, Xiao Z, Yang Q, Yu T, Deng X, Chen N Mater Today Bio. 2024; 25:100978.

PMID: 38434571 PMC: 10907859. DOI: 10.1016/j.mtbio.2024.100978.

Gelatin coating enhances therapeutic cell adhesion to the infarcted myocardium via ECM binding.

Davis K, Gottipatti A, Peng H, Donahue R, Chelvarajan L, Cahall C PLoS One. 2022; 17(11):e0277561.

PMID: 36355857 PMC: 9648752. DOI: 10.1371/journal.pone.0277561.

Tissue repair strategies: What we have learned from COVID-19 in the application of MSCs therapy.

Li Y, Ajoolabady A, Dhanasekaran M, Ren J Pharmacol Res. 2022; 182:106334.

PMID: 35779816 PMC: 9242686. DOI: 10.1016/j.phrs.2022.106334.

Nanotechnology in cardiac stem cell therapy: cell modulation, imaging and gene delivery.

Sarathkumar E, Victor M, Menon J, Jibin K, Padmini S, Jayasree R RSC Adv. 2022; 11(55):34572-34588.

PMID: 35494731 PMC: 9043027. DOI: 10.1039/d1ra06404e.

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