MicroRNA Expression Profile of HCT-8 Cells in the Early Phase of Cryptosporidium Parvum Infection

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2019 Jan 16

PMID 30642246

Citations 13

Authors

Chenrong Wang

Limin Liu

Huili Zhu

Lu Zhang

Rongjun Wang

Zhenjie Zhang

Jianying Huang

Sumei Zhang

Fuchun Jian

Changshen Ning

Longxian Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Cryptosporidium parvum is an important zoonotic parasitic disease worldwide, but the molecular mechanisms of the host-parasite interaction are not fully understood. Noncoding microRNAs (miRNAs) are considered key regulators of parasitic diseases. Therefore, we used microarray, qPCR, and bioinformatic analyses to investigate the intestinal epithelial miRNA expression profile after Cryptosporidium parvum infection.

Results: Twenty miRNAs were differentially expressed after infection (four upregulated and 16 downregulated). Further analysis of the differentially expressed miRNAs revealed that many important cellular responses were triggered by Cryptosporidium parvum infection, including cell apoptosis and the inflammatory and immune responses.

Conclusions: This study demonstrates for the first time that the miRNA expression profile of human intestinal epithelium cells is altered by C. parvum infection. This dysregulation of miRNA expression may contribute to the regulation of host biological processes in response to C. parvum infection, including cell apoptosis and the immune responses. These results provide new insight into the regulatory mechanisms of host miRNAs during cryptosporidiosis, which may offer potential targets for future C. parvum control strategies.

Citing Articles

Molecular pathogenesis of Cryptosporidium and advancements in therapeutic interventions.

Lu Y, Zhang X, Guan Z, Ji R, Peng F, Zhao C Parasite. 2025; 32:7.

PMID: 39902829 PMC: 11792522. DOI: 10.1051/parasite/2025001.

MiR-199a-3p regulates HCT-8 cell autophagy and apoptosis in response to Cryptosporidium parvum infection by targeting MTOR.

Wu S, Shao T, Xie J, Li J, Sun L, Zhang Y Commun Biol. 2024; 7(1):924.

PMID: 39085368 PMC: 11291649. DOI: 10.1038/s42003-024-06632-5.

MiR-3976 regulates HCT-8 cell apoptosis and parasite burden by targeting BCL2A1 in response to Cryptosporidium parvum infection.

Li J, Sun L, Xie F, Shao T, Wu S, Li X Parasit Vectors. 2023; 16(1):221.

PMID: 37415254 PMC: 10324190. DOI: 10.1186/s13071-023-05826-w.

Early immune and host cell responses to infection.

Mead J Front Parasitol. 2023; 2.

PMID: 37325809 PMC: 10269812. DOI: 10.3389/fpara.2023.1113950.

A Systematic Review of Apicomplexa Looking into Epigenetic Pathways and the Opportunity for Novel Therapies.

Brandao Y, Molento M Pathogens. 2023; 12(2).

PMID: 36839571 PMC: 9963874. DOI: 10.3390/pathogens12020299.

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