A New Revision of the Sequence of Plasmid PBR322

Overview

Journal Gene

Specialty Molecular Biology

Date 1988 Oct 30

PMID 3063608

Citations 51

Authors

N Watson

Affiliations

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Abstract

A revised sequence in the region immediately upstream from the rop gene of pBR322 is reported. Two base pairs in the accepted sequence do not exist in the plasmid DNA. Specifically, a TA base pair is missing at sequence coordinate 1893 [Sutcliffe, Cold Spring Harbor Symp. Quant. Biol. 43 (1979) 77-90] and an AT base pair is missing at position 1915, giving a total size for pBR322 of 4361 bp. These changes are in a potential translation initiation sequence and probably reflect errors in the original sequence rather than recent evolution of the plasmid.

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