Human Papilloma Virus Identification in Ocular Surface Squamous Neoplasia by P16 Immunohistochemistry and DNA Chip Test: A Strobe-compliant Article

Overview

Journal Medicine (Baltimore)

Specialty General Medicine

Date 2019 Jan 12

PMID 30633172

Citations 7

Authors

Tina Shrestha

Won Choi

Ga Eon Kim

Jee Myung Yang

Kyung Chul Yoon

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to identify the association between human papilloma virus (HPV) infection and ocular surface squamous neoplasia (OSSN) using p16 immunohistochemistry (IHC) and deoxyribonucleic acid (DNA) chip test.Thirty-eight patients who underwent surgical excision of OSSN were retrospectively studied using tissue samples. The IHC was performed to assess the expression of p16 and DNA chip test was used to detect 24 HPV serotypes.Among the 38 OSSN samples, 32 cases (84.2%) were histopathologically categorized as pre-invasive type and 6 cases (15.8%) as invasive type. The IHC for p16 showed strong positivity in 12 cases (31.6%), whereas it was negative in 26 cases (68.4%). On the other hand, only one case (2.6%) of invasive OSSN was positive for the HPV16 serotype, as assessed by DNA chip test.In OSSN, p16 expression was positive in approximately 1/3rd of the cases, whereas the majority of the 24 HPV serotypes were negative for p16. Our findings suggest that only a weak association exists between HPV infection and OSSN.

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References

Scheffner M, Huibregtse J, Vierstra R, Howley P . The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53. Cell. 1993; 75(3):495-505. DOI: 10.1016/0092-8674(93)90384-3. View

El-Naggar A, Westra W . p16 expression as a surrogate marker for HPV-related oropharyngeal carcinoma: a guide for interpretative relevance and consistency. Head Neck. 2011; 34(4):459-61. DOI: 10.1002/hed.21974. View

Woods M, Chow S, Heng B, Glenn W, Whitaker N, Waring D . Detecting human papillomavirus in ocular surface diseases. Invest Ophthalmol Vis Sci. 2013; 54(13):8069-78. DOI: 10.1167/iovs.13-13140. View

Di Girolamo N, Coroneo M, Wakefield D . UVB-elicited induction of MMP-1 expression in human ocular surface epithelial cells is mediated through the ERK1/2 MAPK-dependent pathway. Invest Ophthalmol Vis Sci. 2003; 44(11):4705-14. DOI: 10.1167/iovs.03-0356. View

Mwololo A, Nyagol J, Rogena E, Ochuk W, Kimani M, Onyango N . Correlation of EGFR, pEGFR and p16INK4 expressions and high risk HPV infection in HIV/AIDS-related squamous cell carcinoma of conjunctiva. Infect Agent Cancer. 2014; 9(1):7. PMC: 3996052. DOI: 10.1186/1750-9378-9-7. View

Rositch A, Gravitt P, Smith J . Growing evidence that HPV infection is associated with an increase in HIV acquisition: exploring the issue of HPV vaccination. Sex Transm Infect. 2013; 89(5):357. DOI: 10.1136/sextrans-2012-050870. View

Coroneo M . Ultraviolet radiation and the anterior eye. Eye Contact Lens. 2011; 37(4):214-24. DOI: 10.1097/ICL.0b013e318223394e. View

Ang K, Harris J, Wheeler R, Weber R, Rosenthal D, Nguyen-Tan P . Human papillomavirus and survival of patients with oropharyngeal cancer. N Engl J Med. 2010; 363(1):24-35. PMC: 2943767. DOI: 10.1056/NEJMoa0912217. View

Lee G, Hirst L . Ocular surface squamous neoplasia. Surv Ophthalmol. 1995; 39(6):429-50. DOI: 10.1016/s0039-6257(05)80054-2. View

10.

Dyson N, Howley P, Munger K, Harlow E . The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science. 1989; 243(4893):934-7. DOI: 10.1126/science.2537532. View

11.

Di Girolamo N . Association of human papilloma virus with pterygia and ocular-surface squamous neoplasia. Eye (Lond). 2011; 26(2):202-11. PMC: 3272209. DOI: 10.1038/eye.2011.312. View

12.

Gichuhi S, Ohnuma S, Sagoo M, Burton M . Pathophysiology of ocular surface squamous neoplasia. Exp Eye Res. 2014; 129():172-82. PMC: 4726664. DOI: 10.1016/j.exer.2014.10.015. View

13.

Bishop J, Lewis Jr J, Rocco J, Faquin W . HPV-related squamous cell carcinoma of the head and neck: An update on testing in routine pathology practice. Semin Diagn Pathol. 2015; 32(5):344-51. DOI: 10.1053/j.semdp.2015.02.013. View

14.

Scott I, Karp C, Nuovo G . Human papillomavirus 16 and 18 expression in conjunctival intraepithelial neoplasia. Ophthalmology. 2002; 109(3):542-7. DOI: 10.1016/s0161-6420(01)00991-5. View

15.

Ogun G, Ogun O, Bekibele C, Akang E . Intraepithelial and invasive squamous neoplasms of the conjunctiva in Ibadan, Nigeria: a clinicopathological study of 46 cases. Int Ophthalmol. 2008; 29(5):401-9. DOI: 10.1007/s10792-008-9257-8. View

16.

Afrogheh A, Jakobiec F, Hammon R, Grossniklaus H, Rocco J, Lindeman N . Evaluation for High-risk HPV in Squamous Cell Carcinomas and Precursor Lesions Arising in the Conjunctiva and Lacrimal Sac. Am J Surg Pathol. 2016; 40(4):519-28. DOI: 10.1097/PAS.0000000000000581. View

17.

Manderwad G, Kannabiran C, Honavar S, Vemuganti G . Lack of association of high-risk human papillomavirus in ocular surface squamous neoplasia in India. Arch Pathol Lab Med. 2009; 133(8):1246-50. DOI: 10.5858/133.8.1246. View

18.

Yeo M, Lee A, Hur S, Park J . Clinical Significance of an HPV DNA Chip Test with Emphasis on HPV-16 and/or HPV-18 Detection in Korean Gynecological Patients. J Pathol Transl Med. 2016; 50(4):294-9. PMC: 4963975. DOI: 10.4132/jptm.2016.05.09. View

19.

Guthoff R, Marx A, Stroebel P . No evidence for a pathogenic role of human papillomavirus infection in ocular surface squamous neoplasia in Germany. Curr Eye Res. 2009; 34(8):666-71. DOI: 10.1080/02713680903007162. View

20.

Kobalka P, Abboud J, Liao X, Jones K, Lee B, Korn B . p16INK4A expression is frequently increased in periorbital and ocular squamous lesions. Diagn Pathol. 2015; 10:175. PMC: 4581440. DOI: 10.1186/s13000-015-0396-8. View