Milestones Achieved in Response to Drought Stress Through Reverse Genetic Approaches

Overview

Journal F1000Res

Specialties Biomedical Engineering
Science

Date 2019 Jan 12

PMID 30631439

Citations 6

Authors

Baljeet Singh

Sarvjeet Kukreja

Umesh Goutam

Affiliations

Soon will be listed here.

Abstract

Drought stress is the most important abiotic stress that constrains crop production and reduces yield drastically. The germplasm of most of the cultivated crops possesses numerous unknown drought stress tolerant genes. Moreover, there are many reports suggesting that the wild species of most of the modern cultivars have abiotic stress tolerant genes. Due to climate change and population booms, food security has become a global issue. To develop drought tolerant crop varieties knowledge of various genes involved in drought stress is required. Different reverse genetic approaches such as virus-induced gene silencing (VIGS), clustered regularly interspace short palindromic repeat (CRISPR), targeting induced local lesions in genomes (TILLING) and expressed sequence tags (ESTs) have been used extensively to study the functionality of different genes involved in response to drought stress. In this review, we described the contributions of different techniques of functional genomics in the study of drought tolerant genes.

Citing Articles

Identification of a highly drought-resistant mutant.

Xu D, Leister D, Kleine T Front Plant Sci. 2024; 15:1341576.

PMID: 38887464 PMC: 11180769. DOI: 10.3389/fpls.2024.1341576.

Response of the organellar and nuclear (post)transcriptomes of Arabidopsis to drought.

Xu D, Tang Q, Xu P, Schaffner A, Leister D, Kleine T Front Plant Sci. 2023; 14:1220928.

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High-Throughput Analysis of Gene Function under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants.

Yamunarani R, Ramegowda V, Senthil-Kumar M, Mysore K Methods Mol Biol. 2022; 2408:181-189.

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Potato biofortification: an effective way to fight global hidden hunger.

Singh B, Goutam U, Kukreja S, Sharma J, Sood S, Bhardwaj V Physiol Mol Biol Plants. 2021; 27(10):2297-2313.

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CRISPR-Cas9-based genetic engineering for crop improvement under drought stress.

Sami A, Xue Z, Tazein S, Arshad A, Zhu Z, Chen Y Bioengineered. 2021; 12(1):5814-5829.

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