Development of a Duplex SYBR Green I-based Quantitative Real-time PCR Assay for the Rapid Differentiation of Goose and Muscovy Duck Parvoviruses

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2019 Jan 12

PMID 30630503

Citations 3

Authors

Su Lin

Shao Wang

Xiaoxia Cheng

Shifeng Xiao

Xiuqin Chen

Shilong Chen

Shaoying Chen

Fusong Yu

Affiliations

Soon will be listed here.

Abstract

Background: Waterfowl parvoviruses, including goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), can cause seriously diseases in geese and ducks. Developing a fast and precise diagnosis assay for these two parvoviruses is particularly important.

Results: A duplex SYBR Green I-based quantitative real-time PCR assay was developed for the simultaneous detection and differentiation of GPV and MDPV. The assay yielded melting curves with specific single peak (Tm = 87.3 ± 0.26 °C or Tm = 85.4 ± 0.23 °C) when GPV or MDPV was evaluated, respectively. When both parvoviruses were assessed in one reaction, melting curves with specific double peaks were yielded.

Conclusion: This duplex quantitative RT-PCR can be used to rapid identify of GPV and MDPV in field cases and artificial trials, which make it a powerful tool for diagnosing, preventing and controlling waterfowl parvovirus infections.

Citing Articles

Development and application of quadruplex real time quantitative PCR method for differentiation of Muscovy duck parvovirus, Goose parvovirus, Duck circovirus, and Duck adenovirus 3.

Wang H, Chen J, An T, Chen H, Wang Y, Zhu L Front Cell Infect Microbiol. 2024; 14:1448480.

PMID: 39224701 PMC: 11366709. DOI: 10.3389/fcimb.2024.1448480.

Development and Evaluation of NanoPCR for the Detection of Goose Parvovirus.

Ma H, Gao X, Fu J, Xue H, Song Y, Zhu K Vet Sci. 2022; 9(9).

PMID: 36136676 PMC: 9506417. DOI: 10.3390/vetsci9090460.

Simultaneous detection of duck circovirus and novel goose parvovirus via SYBR green I-based duplex real-time polymerase chain reaction analysis.

Wang Y, Cui Y, Li Y, Jiang S, Liu H, Wang J Mol Cell Probes. 2020; 53:101648.

PMID: 32798710 PMC: 7426261. DOI: 10.1016/j.mcp.2020.101648.

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