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The Unusually Long Amino Acid Acceptor Stem of Escherichia Coli Selenocysteine TRNA Results from Abnormal Cleavage by RNase P

Overview
Journal Nucleic Acids Res
Publisher Oxford University Press
Specialty Biochemistry
Date 1988 Dec 23
PMID 3062578
Citations 15
Authors
U Burkard
D Soll
Affiliations
Soon will be listed here.
Abstract

The nucleotide sequence of the gene encoding the Escherichia coli selenocysteine tRNA (tRNA(SeCys] predicts an unusually long acceptor stem of 8 base pairs (one more than other tRNAs). Here we show by in vivo experiments (Northern blots, primer extension analysis) and by in vitro RNA processing studies that E. coli tRNA(SeCys) does contain this additional basepair, and that its formation results from abnormal cleavage by RNase P.

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