Anticancer Activity of the Goat Antimicrobial Peptide ChMAP-28

Overview

Journal Front Pharmacol

Date 2019 Jan 10

PMID 30622471

Citations 10

Authors

Anna A Emelianova

Denis V Kuzmin

Pavel V Panteleev

Maxim Sorokin

Anton A Buzdin

Tatiana V Ovchinnikova

Affiliations

Soon will be listed here.

Abstract

Cytotoxic effect of the antimicrobial peptide ChMAP-28 from leucocytes of the goat was examined against five cancer and two normal human cell lines. ChMAP-28 has the amino acid sequence GRFKRFRKKLKRLWHKVGPFVGPILHY and is homologous to other α-helical mammalian antimicrobial peptides. ChMAP-28 shows considerably higher cytotoxicity against cultured tumor cells than toward normal cells at concentrations of <10 μM. Our findings suggest that ChMAP-28 can initiate necrotic death of cancer cells. Its cytotoxic effect is accomplished due to disruption of the plasma membrane integrity and is not abrogated by the addition of the caspase inhibitor Z-VAD-FMK. ChMAP-28 causes permeabilization of cytoplasmic membrane of human leukemia cells HL-60 already after 15 min of incubation. Here, we show that ChMAP-28 has one of the highest antitumor activity among all known antimicrobial peptides. We speculate that the observed specificity of ChMAP-28 cytotoxic effect against tumor cells is due to its relatively low hydrophobicity and high cationicity. In the meantime, this peptide has low hemolytic activity, which generates a potential for its use as a therapeutic agent.

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