Online Photochemical Derivatization Enables Comprehensive Mass Spectrometric Analysis of Unsaturated Phospholipid Isomers

Overview

Journal Nat Commun

Specialty Biology

Date 2019 Jan 10

PMID 30622271

Citations 61

Authors

Wenpeng Zhang

Donghui Zhang

Qinhua Chen

Junhan Wu

Zheng Ouyang

Yu Xia

Affiliations

Soon will be listed here.

Abstract

Mass spectrometry-based lipidomics is the primary tool for the structural analysis of lipids but the effective localization of carbon-carbon double bonds (C=C) in unsaturated lipids to distinguish C=C location isomers remains challenging. Here, we develop a large-scale lipid analysis platform by coupling online C=C derivatization through the Paternò-Büchi reaction with liquid chromatography-tandem mass spectrometry. This provides rich information on lipid C=C location isomers, revealing C=C locations for more than 200 unsaturated glycerophospholipids in bovine liver among which we identify 55 groups of C=C location isomers. By analyzing tissue samples of patients with breast cancer and type 2 diabetes plasma samples, we find that the ratios of C=C isomers are much less affected by interpersonal variations than their individual abundances, suggesting that isomer ratios may be used for the discovery of lipid biomarkers.

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