Genetic Loci Associated With Fluoride Resistance in

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Jan 9

PMID 30619172

Citations 10

Authors

Ying Liao

Jingmei Yang

Bernd W Brandt

Jiyao Li

Wim Crielaard

Cor van Loveren

Dong Mei Deng

Affiliations

Soon will be listed here.

Abstract

The prolonged exposure of the cariogenic bacterial species to high concentrations of fluoride leads to the development of fluoride resistance in this species. Previous studies confirmed the involvement of a mutation in a single chromosomal region in the occurrence of fluoride resistance. The involvement of multiple genomic mutations has not been verified. The aim of this study is to identify multiple genetic loci associated with fluoride resistance in . The previously published whole genome sequences of two fluoride-resistant strains (UA159-FR and C180-2FR) and their corresponding wild-type strains (UA159 and C180-2) were analyzed to locate shared chromosomal mutations in fluoride-resistant strains. Both fluoride-resistant strains were isolated in laboratory by culturing their mother strains in media with high concentrations of fluoride. The corresponding gene expression and enzyme activities were accordingly validated. Mutations were identified in two glycolytic enzymes, namely pyruvate kinase and enolase. Pyruvate kinase was deactivated in fluoride-resistant strain C180-2FR. Enolase was less inhibited by fluoride in fluoride-resistant strain UA159-FR than in its wild-type strain. Mutations in the promoter constitutively increased the promoter activity and up-regulated the expression of the downstream fluoride antiporters in fluoride-resistant strains. Mutations in the intergenic region led to lower expression of , encoding a glycerol uptake facilitator protein, in fluoride-resistant strains than in wild-type strains. Our results revealed that there is overlap of chromosomal regions with mutations among different fluoride-resistant strains. They provide novel candidates for the study of the mechanisms of fluoride resistance.

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References

Valentini G, Chiarelli L, Fortin R, Speranza M, Galizzi A, Mattevi A . The allosteric regulation of pyruvate kinase. J Biol Chem. 2000; 275(24):18145-52. DOI: 10.1074/jbc.M001870200. View

Burne R, Marquis R . Alkali production by oral bacteria and protection against dental caries. FEMS Microbiol Lett. 2000; 193(1):1-6. DOI: 10.1111/j.1574-6968.2000.tb09393.x. View

Sheng J, Liu Z . Acidogenicity and acidurance of fluoride-resistant Streptococcus sobrinus in vitro. Chin J Dent Res. 2001; 3(2):7-14. View

Yoshihara A, Sakuma S, Kobayashi S, Miyazaki H . Antimicrobial effect of fluoride mouthrinse on mutans streptococci and lactobacilli in saliva. Pediatr Dent. 2001; 23(2):113-7. View

Smoot L, Smoot J, Graham M, Somerville G, Sturdevant D, Migliaccio C . Global differential gene expression in response to growth temperature alteration in group A Streptococcus. Proc Natl Acad Sci U S A. 2001; 98(18):10416-21. PMC: 56975. DOI: 10.1073/pnas.191267598. View

Ajdic D, McShan W, McLaughlin R, Savic G, Chang J, Carson M . Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc Natl Acad Sci U S A. 2002; 99(22):14434-9. PMC: 137901. DOI: 10.1073/pnas.172501299. View

Ng P, Henikoff S . SIFT: Predicting amino acid changes that affect protein function. Nucleic Acids Res. 2003; 31(13):3812-4. PMC: 168916. DOI: 10.1093/nar/gkg509. View

Ten Cate J . Fluorides in caries prevention and control: empiricism or science. Caries Res. 2004; 38(3):254-7. DOI: 10.1159/000077763. View

Darling A, Mau B, Blattner F, Perna N . Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res. 2004; 14(7):1394-403. PMC: 442156. DOI: 10.1101/gr.2289704. View

10.

Brenot A, King K, Caparon M . The PerR regulon in peroxide resistance and virulence of Streptococcus pyogenes. Mol Microbiol. 2004; 55(1):221-34. DOI: 10.1111/j.1365-2958.2004.04370.x. View

11.

Haenni M, Moreillon P, Lazarevic V . Promoter and transcription analysis of penicillin-binding protein genes in Streptococcus gordonii. Antimicrob Agents Chemother. 2007; 51(8):2774-83. PMC: 1932516. DOI: 10.1128/AAC.01127-06. View

12.

van Loveren C, Spitz L, Buijs J, Ten Cate J, Eisenberg A . In vitro demineralization of enamel by F-sensitive and F-resistant mutans streptococci in the presence of 0, 0.05, or 0.5 mmol/L NaF. J Dent Res. 1991; 70(12):1491-6. DOI: 10.1177/00220345910700120401. View

13.

van Loveren C, Hoogenkamp M, Deng D, Ten Cate J . Effects of different kinds of fluorides on enolase and ATPase activity of a fluoride-sensitive and fluoride-resistant Streptococcus mutans strain. Caries Res. 2008; 42(6):429-34. DOI: 10.1159/000159606. View

14.

Darling A, Mau B, Perna N . progressiveMauve: multiple genome alignment with gene gain, loss and rearrangement. PLoS One. 2010; 5(6):e11147. PMC: 2892488. DOI: 10.1371/journal.pone.0011147. View

15.

Zoraghi R, See R, Gong H, Lian T, Swayze R, Finlay B . Functional analysis, overexpression, and kinetic characterization of pyruvate kinase from methicillin-resistant Staphylococcus aureus. Biochemistry. 2010; 49(35):7733-47. DOI: 10.1021/bi100780t. View

16.

Yoneyama H, Hori H, Lim S, Murata T, Ando T, Isogai E . Isolation of a mutant auxotrophic for L-alanine and identification of three major aminotransferases that synthesize L-alanine in Escherichia coli. Biosci Biotechnol Biochem. 2011; 75(5):930-8. DOI: 10.1271/bbb.100905. View

17.

Zhu L, Zhang Z, Liang J . Fatty-acid profiles and expression of the fabM gene in a fluoride-resistant strain of Streptococcus mutans. Arch Oral Biol. 2011; 57(1):10-4. DOI: 10.1016/j.archoralbio.2011.06.011. View

18.

Baker J, Sudarsan N, Weinberg Z, Roth A, Stockbridge R, Breaker R . Widespread genetic switches and toxicity resistance proteins for fluoride. Science. 2011; 335(6065):233-235. PMC: 4140402. DOI: 10.1126/science.1215063. View

19.

Naumova E, Sandulescu T, Bochnig C, Gaengler P, Zimmer S, Arnold W . Kinetics of fluoride bioavailability in supernatant saliva and salivary sediment. Arch Oral Biol. 2012; 57(7):870-6. DOI: 10.1016/j.archoralbio.2012.01.011. View

20.

Lemos J, Quivey R, Koo H, Abranches J . Streptococcus mutans: a new Gram-positive paradigm?. Microbiology (Reading). 2013; 159(Pt 3):436-445. PMC: 4083656. DOI: 10.1099/mic.0.066134-0. View