A Remote -Regulatory Region Is Required for Expression in the Pericycle to Initiate Nodule Primordium Formation in

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 2019 Jan 6

PMID 30610167

Citations 57

Authors

Jieyu Liu

Luuk Rutten

Erik Limpens

Tjitse van der Molen

Robin van Velzen

Rujin Chen

Yuhui Chen

Rene Geurts

Wouter Kohlen

Olga Kulikova

Ton Bisseling

Affiliations

Soon will be listed here.

Abstract

The legume-rhizobium symbiosis results in nitrogen-fixing root nodules, and their formation involves both intracellular infection initiated in the epidermis and nodule organogenesis initiated in inner root cell layers. () is a nodule-specific transcription factor essential for both processes. These NIN-regulated processes occur at different times and locations in the root, demonstrating a complex pattern of spatiotemporal regulation. We show that regulatory sequences sufficient for the epidermal infection process are located within a 5 kb region directly upstream of the start codon in Furthermore, we identify a remote upstream -regulatory region required for the expression of in the pericycle, and we show that this region is essential for nodule organogenesis. This region contains putative cytokinin response elements and is conserved in eight more legume species. Both the cytokinin receptor 1, which is essential for nodule primordium formation, and the B-type response regulator are expressed in the pericycle in the susceptible zone of the uninoculated root. This, together with the identification of the cytokinin-responsive elements in the promoter, strongly suggests that expression is initially triggered by cytokinin signaling in the pericycle to initiate nodule primordium formation.

Citing Articles

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