Overexpression of the Jojoba Aquaporin Gene, , Enhances Drought and Salt Tolerance in Transgenic

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Jan 6

PMID 30609831

Citations 26

Authors

Xing Wang

Fei Gao

Jie Bing

Weimin Sun

Xiuxiu Feng

Xiaofeng Ma

Yijun Zhou

Genfa Zhang

Affiliations

Soon will be listed here.

Abstract

Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporin proteins located on plasma membranes where they facilitate the transport of water and small uncharged solutes. PIPs play an important role throughout plant development, and in response to abiotic stresses. Jojoba ( (Link) Schneider), as a typical desert plant, tolerates drought, salinity and nutrient-poor soils. In this study, a gene () was cloned from jojoba and overexpressed in . The expression of at the transcriptional level was induced by polyethylene glycol (PEG) treatment. overexpressed plants exhibited higher germination rates, longer roots and higher survival rates compared to the wild-type plants under drought and salt stresses. The results of malonaldehyde (MDA), ion leakage (IL) and proline content measurements indicated that the improved drought and salt tolerance conferred by was correlated with decreased membrane damage and improved osmotic adjustment. We assume that may be applied to genetic engineering to improve plant tolerance based on the resistance effect in transgenic overexpressing .

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