An Experimental Study on the Mechanical and Biological Properties of Bio-Printed Alginate/Halloysite Nanotube/Methylcellulose/Russian Olive-Based Scaffolds

Overview

Journal Adv Pharm Bull

Date 2019 Jan 5

PMID 30607337

Citations 6

Authors

Babak Roushangar Zineh

Mohammad Reza Shabgard

Leila Roshangar

Affiliations

Soon will be listed here.

Abstract

Cartilage shows neither repairs nor regenerative properties after trauma or gradual wear and causes severe pain due to bones rubbing. Bioprinting of tissue-engineered artificial cartilage is one of the most fast-growing sciences in this area that can help millions of people against this disease. Bioprinting of proper bioscaffolds for cartilage repair was the main goal of this study. The bioprinting process was achieved by a novel composition consisting of alginate (AL), Halloysite nanotube (HNT), and methylcellulose (MC) prepared in bio-ink. Also, the effect of Russian olive (RO) in chondrocytes growth on bioscaffolds was also investigated in this work. Compressive, hardness and viscosity tests, Energy-Dispersive X-Ray Spectroscopy (EDX), Fourier-Transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC), water-soluble Tetrazolium (WST) assay, and also transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were carried out. The results show that in constant concentrations of AL, MC, and RO (20 mg/ml AL, 20 mg/ml MC, and 10 mg/ml RO) when concentration of HNT increased from 10 mg/ml (T-7) to 20 mg/ml (T-8) compressive stiffness increased from 241±45 kPa to 500.66±19.50 kPa. Also, 20 mg/ml of AL in composition saved proper water content for chondrocyte growth and produced good viscosity properties for a higher printing resolution. RO increased chondrocytes living cell efficiency by 11% on bioprinted scaffolds in comparison with the control group without RO. Results obtained through in-vivo studies were similar to those of in-vitro studies. According to the results, T-7 bio-ink has good potential in bioprinting of scaffolds in cartilage repairs.

Citing Articles

3D printing of alginate/thymoquinone/halloysite nanotube bio-scaffolds for cartilage repairs: experimental and numerical study.

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PMID: 36066743 DOI: 10.1007/s11517-022-02654-5.

A comprehensive review on methods for promotion of mechanical features and biodegradation rate in amniotic membrane scaffolds.

Sarvari R, Keyhanvar P, Agbolaghi S, Roshangar L, Bahremani E, Keyhanvar N J Mater Sci Mater Med. 2022; 33(3):32.

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Fabrication and characterization of microstructure-controllable COL-HA-PVA hydrogels for cartilage repair.

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Tailorable Zinc-Substituted Mesoporous Bioactive Glass/Alginate-Methylcellulose Composite Bioinks.

Guduric V, Belton N, Richter R, Bernhardt A, Spangenberg J, Wu C Materials (Basel). 2021; 14(5).

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References

Samiei M, Aghazadeh M, Alizadeh E, Aslaminabadi N, Davaran S, Shirazi S . Osteogenic/Odontogenic Bioengineering with co-Administration of Simvastatin and Hydroxyapatite on Poly Caprolactone Based Nanofibrous Scaffold. Adv Pharm Bull. 2016; 6(3):353-365. PMC: 5071798. DOI: 10.15171/apb.2016.047. View

Rhee S, Puetzer J, Mason B, Reinhart-King C, Bonassar L . 3D Bioprinting of Spatially Heterogeneous Collagen Constructs for Cartilage Tissue Engineering. ACS Biomater Sci Eng. 2021; 2(10):1800-1805. DOI: 10.1021/acsbiomaterials.6b00288. View

Farzaei M, Bahramsoltani R, Abbasabadi Z, Rahimi R . A comprehensive review on phytochemical and pharmacological aspects of Elaeagnus angustifolia L. J Pharm Pharmacol. 2015; 67(11):1467-80. DOI: 10.1111/jphp.12442. View

Tehranizadeh Z, Baratian A, Hosseinzadeh H . Russian olive () as a herbal healer. Bioimpacts. 2016; 6(3):155-167. PMC: 5108988. DOI: 10.15171/bi.2016.22. View

Markstedt K, Mantas A, Tournier I, Martinez Avila H, Hagg D, Gatenholm P . 3D Bioprinting Human Chondrocytes with Nanocellulose-Alginate Bioink for Cartilage Tissue Engineering Applications. Biomacromolecules. 2015; 16(5):1489-96. DOI: 10.1021/acs.biomac.5b00188. View

Li H, Tan Y, Leong K, Li L . 3D Bioprinting of Highly Thixotropic Alginate/Methylcellulose Hydrogel with Strong Interface Bonding. ACS Appl Mater Interfaces. 2017; 9(23):20086-20097. DOI: 10.1021/acsami.7b04216. View

Akizuki S, Mow V, Muller F, Pita J, Howell D, Manicourt D . Tensile properties of human knee joint cartilage: I. Influence of ionic conditions, weight bearing, and fibrillation on the tensile modulus. J Orthop Res. 1986; 4(4):379-92. DOI: 10.1002/jor.1100040401. View

Panwar A, Tan L . Current Status of Bioinks for Micro-Extrusion-Based 3D Bioprinting. Molecules. 2016; 21(6). PMC: 6273655. DOI: 10.3390/molecules21060685. View

Liu M, Dai L, Shi H, Xiong S, Zhou C . In vitro evaluation of alginate/halloysite nanotube composite scaffolds for tissue engineering. Mater Sci Eng C Mater Biol Appl. 2015; 49:700-712. DOI: 10.1016/j.msec.2015.01.037. View

10.

Chung J, Naficy S, Yue Z, Kapsa R, Quigley A, Moulton S . Bio-ink properties and printability for extrusion printing living cells. Biomater Sci. 2020; 1(7):763-773. DOI: 10.1039/c3bm00012e. View

11.

Hamidpour R, Hamidpour S, Hamidpour M, Shahlari M, Sohraby M, Shahlari N . Russian olive ( L.): From a variety of traditional medicinal applications to its novel roles as active antioxidant, anti-inflammatory, anti-mutagenic and analgesic agent. J Tradit Complement Med. 2017; 7(1):24-29. PMC: 5198788. DOI: 10.1016/j.jtcme.2015.09.004. View

12.

Li X, Dunne N, Li X, Aifantis K . Scaffolds reinforced by fibers or tubes for tissue repair. Biomed Res Int. 2014; 2014:501275. PMC: 4151618. DOI: 10.1155/2014/501275. View

13.

Amir Afshar H, Ghaee A . Preparation of aminated chitosan/alginate scaffold containing halloysite nanotubes with improved cell attachment. Carbohydr Polym. 2016; 151:1120-1131. DOI: 10.1016/j.carbpol.2016.06.063. View

14.

Pritzker K, Gay S, Jimenez S, Ostergaard K, Pelletier J, Revell P . Osteoarthritis cartilage histopathology: grading and staging. Osteoarthritis Cartilage. 2005; 14(1):13-29. DOI: 10.1016/j.joca.2005.07.014. View

15.

Kang H, Lee S, Ko I, Kengla C, Yoo J, Atala A . A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nat Biotechnol. 2016; 34(3):312-9. DOI: 10.1038/nbt.3413. View

16.

Ahmadi M, Rahbarghazi R, Aslani M, Shahbazfar A, Kazemi M, Keyhanmanesh R . Bone marrow mesenchymal stem cells and their conditioned media could potentially ameliorate ovalbumin-induced asthmatic changes. Biomed Pharmacother. 2016; 85:28-40. DOI: 10.1016/j.biopha.2016.11.127. View

17.

Fakhrullin R, Lvov Y . Halloysite clay nanotubes for tissue engineering. Nanomedicine (Lond). 2016; 11(17):2243-6. DOI: 10.2217/nnm-2016-0250. View

18.

Roth V, Mow V . The intrinsic tensile behavior of the matrix of bovine articular cartilage and its variation with age. J Bone Joint Surg Am. 1980; 62(7):1102-17. View

19.

Lotfipour F, Mirzaeei S, Maghsoodi M . Evaluation of the effect of CaCl2 and alginate concentrations and hardening time on the characteristics of Lactobacillus acidophilus loaded alginate beads using response surface analysis. Adv Pharm Bull. 2013; 2(1):71-8. PMC: 3846017. DOI: 10.5681/apb.2012.010. View

20.

Camarero-Espinosa S, Rothen-Rutishauser B, Foster E, Weder C . Articular cartilage: from formation to tissue engineering. Biomater Sci. 2016; 4(5):734-67. DOI: 10.1039/c6bm00068a. View