Photoacoustic Microscopy: Principles and Biomedical Applications

Overview

Journal Biomed Eng Lett

Specialty Biomedical Engineering

Date 2019 Jan 4

PMID 30603203

Citations 37

Authors

Wei Liu

Junjie Yao

Affiliations

Soon will be listed here.

Abstract

Photoacoustic microscopy (PAM) has become an increasingly popular technology for biomedical applications, providing anatomical, functional, and molecular information. In this concise review, we first introduce the basic principles and typical system designs of PAM, including optical-resolution PAM and acoustic-resolution PAM. The major imaging characteristics of PAM, i.e. spatial resolutions, penetration depth, and scanning approach are discussed in detail. Then, we introduce the major biomedical applications of PAM, including anatomical imaging across scales from cellular level to organismal level, label-free functional imaging using endogenous biomolecules, and molecular imaging using exogenous contrast agents. Lastly, we discuss the technical and engineering challenges of PAM in the translation to potential clinical impacts.

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References

Schnackenberg C . Physiological and pathophysiological roles of oxygen radicals in the renal microvasculature. Am J Physiol Regul Integr Comp Physiol. 2002; 282(2):R335-42. DOI: 10.1152/ajpregu.00605.2001. View

Amann K, Tornig J, Kugel B, Gross M, Tyralla K, El-Shakmak A . Hyperphosphatemia aggravates cardiac fibrosis and microvascular disease in experimental uremia. Kidney Int. 2003; 63(4):1296-301. DOI: 10.1046/j.1523-1755.2003.00864.x. View

Miles K, Griffiths M, Keith C . Blood flow-metabolic relationships are dependent on tumour size in non-small cell lung cancer: a study using quantitative contrast-enhanced computer tomography and positron emission tomography. Eur J Nucl Med Mol Imaging. 2005; 33(1):22-8. DOI: 10.1007/s00259-005-1932-7. View

Zhang H, Maslov K, Stoica G, Wang L . Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging. Nat Biotechnol. 2006; 24(7):848-51. DOI: 10.1038/nbt1220. View

Andersen H, Lanng S, Pressler T, Laugesen C, Mathiesen E . Cystic fibrosis-related diabetes: the presence of microvascular diabetes complications. Diabetes Care. 2006; 29(12):2660-3. DOI: 10.2337/dc06-0654. View

Zhang H, Maslov K, Wang L . In vivo imaging of subcutaneous structures using functional photoacoustic microscopy. Nat Protoc. 2007; 2(4):797-804. DOI: 10.1038/nprot.2007.108. View

Foster F, Lockwood G, Ryan L, Harasiewicz K, Berube L, Rauth A . Principles and applications of ultrasound backscatter microscopy. IEEE Trans Ultrason Ferroelectr Freq Control. 1993; 40(5):608-17. DOI: 10.1109/58.238115. View

Maslov K, Zhang H, Hu S, Wang L . Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries. Opt Lett. 2008; 33(9):929-31. DOI: 10.1364/ol.33.000929. View

Zhang E, Laufer J, Pedley R, Beard P . In vivo high-resolution 3D photoacoustic imaging of superficial vascular anatomy. Phys Med Biol. 2009; 54(4):1035-46. DOI: 10.1088/0031-9155/54/4/014. View

10.

Stein E, Maslov K, Wang L . Noninvasive, in vivo imaging of blood-oxygenation dynamics within the mouse brain using photoacoustic microscopy. J Biomed Opt. 2009; 14(2):020502. PMC: 2676448. DOI: 10.1117/1.3095799. View

11.

Zhang H, Maslov K, Li M, Stoica G, Wang L . In vivo volumetric imaging of subcutaneous microvasculature by photoacoustic microscopy. Opt Express. 2009; 14(20):9317-23. DOI: 10.1364/oe.14.009317. View

12.

Yang D, Xing D, Yang S, Xiang L . Fast full-view photoacoustic imaging by combined scanning with a linear transducer array. Opt Express. 2009; 15(23):15566-75. DOI: 10.1364/oe.15.015566. View

13.

Huang D, Swanson E, Lin C, Schuman J, Stinson W, Chang W . Optical coherence tomography. Science. 1991; 254(5035):1178-81. PMC: 4638169. DOI: 10.1126/science.1957169. View

14.

Li P, Wang C, Shieh D, Wei C, Liao C, Poe C . In vivo photoacoustic molecular imaging with simultaneous multiple selective targeting using antibody-conjugated gold nanorods. Opt Express. 2009; 16(23):18605-15. DOI: 10.1364/oe.16.018605. View

15.

Stein E, Maslov K, Wang L . Noninvasive, in vivo imaging of the mouse brain using photoacoustic microscopy. J Appl Phys. 2009; 105(10):102027. PMC: 2719465. DOI: 10.1063/1.3116134. View

16.

Li C, Wang L . Photoacoustic tomography and sensing in biomedicine. Phys Med Biol. 2009; 54(19):R59-97. PMC: 2872141. DOI: 10.1088/0031-9155/54/19/R01. View

17.

Guo Z, Li L, Wang L . On the speckle-free nature of photoacoustic tomography. Med Phys. 2009; 36(9):4084-8. PMC: 2738745. DOI: 10.1118/1.3187231. View

18.

Yao J, Maslov K, Hu S, Wang L . Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo. J Biomed Opt. 2009; 14(5):054049. PMC: 2782364. DOI: 10.1117/1.3251044. View

19.

Wang L . Multiscale photoacoustic microscopy and computed tomography. Nat Photonics. 2010; 3(9):503-509. PMC: 2802217. DOI: 10.1038/nphoton.2009.157. View

20.

Kim C, Favazza C, Wang L . In vivo photoacoustic tomography of chemicals: high-resolution functional and molecular optical imaging at new depths. Chem Rev. 2010; 110(5):2756-82. PMC: 2872199. DOI: 10.1021/cr900266s. View