Improves Metronidazole-induced Impaired Fertility in the Male Mice

Overview

Journal Afr Health Sci

Date 2019 Jan 4

PMID 30602997

Citations 2

Authors

Mrinalini Kumari

Poonam Singh

Affiliations

Soon will be listed here.

Abstract

Introduction: Fruit extract of (TT) bears aphrodisiac and antioxidative properties. Antimicrobial drug, metronidazole (MTZ) impairs the spermatogenic activity and fertility in males.

Objective: Validation of the use of fruit extract of TT as a supplement against MTZ-induced fertility impairment in males.

Methods: Adult Swiss strain male mice were administered with 500mg/kgBW/day of MTZ for 28 days. Low (100mg/kgBW/day) and high (200mg/kgBW/day) doses of TT were administered simultaneously with MTZ (500mg/kgBW/day) for same duration. All males were cohabited with virgin proestrus females. Vaginal plug formation was observed to calculate the libido index. Cohabited females were sacrificed on fifteenth day of gestation to dissect out the ovaries and uteri. Fertility index, quantal pregnancy, pre-implantation and post-implantation losses were calculated.

Results: MTZ-treated males showed unaltered mating ability, however, the females impregnated by such males exhibited marked alterations in the fertility index, quantal pregnancy and pre- and post-implantation losses. Supplementation with low dose of TT failed to restore such reproductive toxicities exhibited by administration of MTZ. However, the altered reproductive toxicities were reinstated to control values following supplementation with high dose of TT.

Conclusion: The fruit extract of TT may emerge as an effective herbal remedy, correcting the drug-induced fertility impairments in males.

Citing Articles

Puncture vine (Tribulus Terrestris L.) in control of health and reproduction.

Sirotkin A, Kolesarova A Physiol Res. 2022; 70(Suppl4):S657-S667.

PMID: 35199550 PMC: 9054192. DOI: 10.33549/physiolres.934711.

Progress in infections, reproductive health and non-communicable diseases.

Tumwine J Afr Health Sci. 2019; 18(3):i-iv.

PMID: 30603019 PMC: 6306991. DOI: 10.4314/ahs.v18i3.1.

References

Achkar J, Hanauer S . Medical therapy to reduce postoperative Crohn's disease recurrence. Am J Gastroenterol. 2000; 95(5):1139-46. DOI: 10.1111/j.1572-0241.2000.02000.x. View

Brown G, Vukovich M, Martini E, Kohut M, Franke W, Jackson D . Endocrine and lipid responses to chronic androstenediol-herbal supplementation in 30 to 58 year old men. J Am Coll Nutr. 2001; 20(5):520-8. DOI: 10.1080/07315724.2001.10719061. View

Grover J, Vats V, Srinivas M, Das S, Jha P, Gupta D . Effect of metronidazole on spermatogenesis and FSH, LH and testosterone levels of pre-pubertal rats. Indian J Exp Biol. 2002; 39(11):1160-2. View

Gauthaman K, Ganesan A, Prasad R . Sexual effects of puncturevine (Tribulus terrestris) extract (protodioscin): an evaluation using a rat model. J Altern Complement Med. 2003; 9(2):257-65. DOI: 10.1089/10755530360623374. View

El-Nahas A, El-Ashmawy I . Reproductive and cytogenetic toxicity of metronidazole in male mice. Basic Clin Pharmacol Toxicol. 2004; 94(5):226-31. DOI: 10.1111/j.1742-7843.2004.pto940505.x. View

Reagan-Shaw S, Nihal M, Ahmad N . Dose translation from animal to human studies revisited. FASEB J. 2007; 22(3):659-61. DOI: 10.1096/fj.07-9574LSF. View

Martino-Andrade A, Morais R, Spercoski K, Rossi S, Vechi M, Golin M . Effects of Tribulus terrestris on endocrine sensitive organs in male and female Wistar rats. J Ethnopharmacol. 2009; 127(1):165-70. DOI: 10.1016/j.jep.2009.09.031. View

Lofmark S, Edlund C, Nord C . Metronidazole is still the drug of choice for treatment of anaerobic infections. Clin Infect Dis. 2010; 50 Suppl 1:S16-23. DOI: 10.1086/647939. View

Riaz A, Khan R, Ahmed S, Afroz S . Assessment of acute toxicity and reproductive capability of a herbal combination. Pak J Pharm Sci. 2010; 23(3):291-4. View

10.

Rajendar B, Bharavi K, Rao G, Kishore P, Ravi Kumar P, Satish Kumar C . Protective effect of an aphrodisiac herb Tribulus terrestris Linn on cadmium-induced testicular damage. Indian J Pharmacol. 2011; 43(5):568-73. PMC: 3195129. DOI: 10.4103/0253-7613.84974. View

11.

Karbalay-Doust S, Noorafshan A . Ameliorative effects of curcumin on the spermatozoon tail length, count, motility and testosterone serum level in metronidazole-treated mice. Prague Med Rep. 2011; 112(4):288-97. View

12.

Singh S, Nair V, Gupta Y . Evaluation of the aphrodisiac activity of Tribulus terrestris Linn. in sexually sluggish male albino rats. J Pharmacol Pharmacother. 2012; 3(1):43-7. PMC: 3284036. DOI: 10.4103/0976-500X.92512. View

13.

Ligha A, Bokolo B, Didia B . Antifertility potentials of metronidazole in male Wistar rats. Pak J Biol Sci. 2013; 15(5):224-30. DOI: 10.3923/pjbs.2012.224.230. View

14.

Kumari M, Singh P . Study on The Reproductive Organs and Fertility of The Male Mice following Administration of Metronidazole. Int J Fertil Steril. 2014; 7(3):225-38. PMC: 3914484. View

15.

Sharma P, Huq A, Singh R . Cypermethrin induced reproductive toxicity in male Wistar rats: protective role of Tribulus terrestris. J Environ Biol. 2014; 34(5):857-62. View

16.

Kumari M, Singh P . Tribulus terrestris ameliorates metronidazole-induced spermatogenic inhibition and testicular oxidative stress in the laboratory mouse. Indian J Pharmacol. 2015; 47(3):304-10. PMC: 4450557. DOI: 10.4103/0253-7613.157129. View

17.

McClain R, Downing J, Edgcomb J . Effect of metronidazole on fertility and testicular function in male rats. Fundam Appl Toxicol. 1989; 12(3):386-96. DOI: 10.1016/0272-0590(89)90014-6. View

18.

Chellman G, Bus J, Working P . Role of epididymal inflammation in the induction of dominant lethal mutations in Fischer 344 rat sperm by methyl chloride. Proc Natl Acad Sci U S A. 1986; 83(21):8087-91. PMC: 386872. DOI: 10.1073/pnas.83.21.8087. View

19.

Mudry M, Carballo M, Labal de Vinuesa M, Gonzalez Cid M, Larripa I . Mutagenic bioassay of certain pharmacological drugs: III. Metronidazole (MTZ). Mutat Res. 1994; 305(2):127-32. DOI: 10.1016/0027-5107(94)90230-5. View

20.

Denker H . Implantation: a cell biological paradox. J Exp Zool. 1993; 266(6):541-58. DOI: 10.1002/jez.1402660606. View