Molecular Surveillance of HCV Mono-infection and HCV-HBV Co-infection in Symptomatic Population at Hyderabad, Pakistan

Overview

Journal Afr Health Sci

Date 2019 Jan 4

PMID 30602984

Citations 1

Authors

Bushra B Patoli

Atif A Patoli

Nimerta Kumari Balani

Ashfaque A Korejo

Affiliations

Soon will be listed here.

Abstract

Background: Pakistan is endemic to hepatitis B and C infections. Alarming rise in hepatitis C virus (HCV) infection has been noticed in some areas of Sindh with an increasing risk for co-infection frequency in this region.

Objective: To estimate the burden of HBV/HCV infection in Hyderabad Pakistan.

Methods: ELISA and Nucleic acid Amplification test were performed to detect viruses. SPSS and online calculator were used for statistical analysis.

Results: From a total of 108 seropositive hepatitis patients, 36.1% (n=39) were found HCV RNA-positive. Non-significant differences were observed in the frequencies of HCV infection for both genders [OR=0.735, CI (95%) 0.307-1.761, p<0.05]. The percentage of HBV DNA detection among 108 HCV-seropositive cases was 17.9% (n=19). However, HCV-HBV co-infection in HCV-RNA positive cases was determined in 48.7% (n=19) cases with non-significant difference in both genders [OR=1.51, CI (95%) = 0.38 - 5.96, p< 0.05]. Analysis suggested weakly positive correlation between HCV mono-infection and HCV-HBV co-infection and age (r =0.184, and r =0.1231), respectively.

Conclusion: The study demonstrates a high prevalence of HBV co-infection among active hepatitis C patients of Hyderabad.

Citing Articles

Progress in infections, reproductive health and non-communicable diseases.

Tumwine J Afr Health Sci. 2019; 18(3):i-iv.

PMID: 30603019 PMC: 6306991. DOI: 10.4314/ahs.v18i3.1.

References

Blakely T, Bates M, Baker M, Tobias M . Hepatitis B carriage explains the excess rate of hepatocellular carcinoma for Maori, Pacific Island and Asian people compared to Europeans in New Zealand. Int J Epidemiol. 1999; 28(2):204-10. DOI: 10.1093/ije/28.2.204. View

Andre F . Hepatitis B epidemiology in Asia, the Middle East and Africa. Vaccine. 2000; 18 Suppl 1:S20-2. DOI: 10.1016/s0264-410x(99)00456-9. View

Lavanchy D . Hepatitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures. J Viral Hepat. 2004; 11(2):97-107. DOI: 10.1046/j.1365-2893.2003.00487.x. View

Liaw Y, Chen Y, Sheen I, Chien R, Yeh C, Chu C . Impact of acute hepatitis C virus superinfection in patients with chronic hepatitis B virus infection. Gastroenterology. 2004; 126(4):1024-9. DOI: 10.1053/j.gastro.2004.01.011. View

Khokhar N, Gill M, Malik G . General seroprevalence of hepatitis C and hepatitis B virus infections in population. J Coll Physicians Surg Pak. 2004; 14(9):534-6. DOI: 09.2004/JCPSP.534536. View

Raimondo G, Brunetto M, Pontisso P, Smedile A, Maina A, Saitta C . Longitudinal evaluation reveals a complex spectrum of virological profiles in hepatitis B virus/hepatitis C virus-coinfected patients. Hepatology. 2005; 43(1):100-7. DOI: 10.1002/hep.20944. View

Jafri W, Jafri N, Yakoob J, Islam M, Tirmizi S, Jafar T . Hepatitis B and C: prevalence and risk factors associated with seropositivity among children in Karachi, Pakistan. BMC Infect Dis. 2006; 6:101. PMC: 1539007. DOI: 10.1186/1471-2334-6-101. View

Alter M . Epidemiology of hepatitis C virus infection. World J Gastroenterol. 2007; 13(17):2436-41. PMC: 4146761. DOI: 10.3748/wjg.v13.i17.2436. View

Alam M, Zaidi S, Malik S, Naeem A, Shaukat S, Sharif S . Serology based disease status of Pakistani population infected with hepatitis B virus. BMC Infect Dis. 2007; 7:64. PMC: 1913529. DOI: 10.1186/1471-2334-7-64. View

10.

Takamizawa A, Mori C, FUKE I, Manabe S, Murakami S, Fujita J . Structure and organization of the hepatitis C virus genome isolated from human carriers. J Virol. 1991; 65(3):1105-13. PMC: 239876. DOI: 10.1128/JVI.65.3.1105-1113.1991. View

11.

Lavanchy D . The global burden of hepatitis C. Liver Int. 2009; 29 Suppl 1:74-81. DOI: 10.1111/j.1478-3231.2008.01934.x. View

12.

Abbas Z, Jeswani N, Kakepoto G, Islam M, Mehdi K, Jafri W . Prevalence and mode of spread of hepatitis B and C in rural Sindh, Pakistan. Trop Gastroenterol. 2009; 29(4):210-6. View

13.

Sarwar J, Gul N, Idris M, Anis-Ur-Rehman , Farid J, Adeel M . Seroprevalence of hepatitis B and hepatitis C in health care workers in Abbottabad. J Ayub Med Coll Abbottabad. 2009; 20(3):27-9. View

14.

Baig S, Siddiqui A, Ahmed W, Qureshi H, Arif A . Frequency of hepatitis C and D super infection in patients with hepatitis B related complex liver disorders. J Coll Physicians Surg Pak. 2009; 19(11):699-703. DOI: 11.2009/JCPSP.699703. View

15.

Waheed Y, Shafi T, Safi S, Qadri I . Hepatitis C virus in Pakistan: a systematic review of prevalence, genotypes and risk factors. World J Gastroenterol. 2009; 15(45):5647-53. PMC: 2789216. DOI: 10.3748/wjg.15.5647. View

16.

Janjua N, Hamza H, Islam M, Tirmizi S, Siddiqui A, Jafri W . Health care risk factors among women and personal behaviours among men explain the high prevalence of hepatitis C virus infection in Karachi, Pakistan. J Viral Hepat. 2009; 17(5):317-26. DOI: 10.1111/j.1365-2893.2009.01230.x. View

17.

Ahmed W, Qureshi H, Arif A, Alam S . Changing trend of viral hepatitis--"A twenty one year report from Pakistan Medical Research Council Research Centre, Jinnah Postgraduate Medical Centre, Karachi". J Pak Med Assoc. 2010; 60(2):86-9. View

18.

Ali M, Idrees M, Ali L, Hussain A, Rehman I, Saleem S . Hepatitis B virus in Pakistan: a systematic review of prevalence, risk factors, awareness status and genotypes. Virol J. 2011; 8:102. PMC: 3058090. DOI: 10.1186/1743-422X-8-102. View

19.

Bosan A, Qureshi H, Bile K, Ahmad I, Hafiz R . A review of hepatitis viral infections in Pakistan. J Pak Med Assoc. 2011; 60(12):1045-58. View

20.

Aziz S, Khanani R, Noorulain W, Rajper J . Frequency of hepatitis B and C in rural and periurban Sindh. J Pak Med Assoc. 2011; 60(10):853-7. View