Diagnostic Deoxyribonucleic Acid Probes for Infectious Diseases

Overview

Journal Clin Microbiol Rev

Publisher American Society for Microbiology

Specialty Microbiology

Date 1988 Jan 1

PMID 3060247

Citations 56

Authors

F C Tenover

Affiliations

Soon will be listed here.

Abstract

Virtually all microorganisms contain some unique nucleotide sequences which can be the target of deoxyribonucleic acid probes. Probes have been used successfully to identify a wide variety of pathogens from the simple ribonucleic acid-containing polioviruses to the complex filarial worms Brugia malayi. Probe technology offers the clinical laboratory the potential both to extend the types of pathogens that can be readily identified and to reduce significantly the time associated with the identification of fastidious microorganisms. Over a dozen commercially prepared deoxyribonucleic acid probe tests are now available. This article explores the development of deoxyribonucleic acid probe tests and reviews the sensitivity, specificity, and predictive values of many of the diagnostic probes developed during the last several years. Prospects for newer, more sensitive detection systems for the products of hybridization reactions are also reviewed.

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References

Ho M, Miller G, ATCHISON R, BREINIG M, Dummer J, Andiman W . Epstein-Barr virus infections and DNA hybridization studies in posttransplantation lymphoma and lymphoproliferative lesions: the role of primary infection. J Infect Dis. 1985; 152(5):876-86. PMC: 3154749. DOI: 10.1093/infdis/152.5.876. View

Wood P, Morris Jr J, Small P, Sethabutr O, Toledo M, TRABULSI L . Comparison of DNA probes and the Sereny test for identification of invasive Shigella and Escherichia coli strains. J Clin Microbiol. 1986; 24(3):498-500. PMC: 268950. DOI: 10.1128/jcm.24.3.498-500.1986. View

Seriwatana J, Echeverria P, Taylor D, Sakuldaipeara T, Changchawalit S, Chivoratanond O . Identification of enterotoxigenic Escherichia coli with synthetic alkaline phosphatase-conjugated oligonucleotide DNA probes. J Clin Microbiol. 1987; 25(8):1438-41. PMC: 269242. DOI: 10.1128/jcm.25.8.1438-1441.1987. View

Jablonski E, Moomaw E, TULLIS R, Ruth J . Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes. Nucleic Acids Res. 1986; 14(15):6115-28. PMC: 311625. DOI: 10.1093/nar/14.15.6115. View

Hyypia T, Stalhandske P, Vainionpaa R, Pettersson U . Detection of enteroviruses by spot hybridization. J Clin Microbiol. 1984; 19(3):436-8. PMC: 271084. DOI: 10.1128/jcm.19.3.436-438.1984. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Blum H, Figus A, Haase A, VYAS G . Laboratory diagnosis of hepatitis B virus infection by nucleic acid hybridization analyses and immunohistologic detection of gene products. Dev Biol Stand. 1985; 59:125-39. View

Guerrant R, Shields D, Thorson S, Schorling J, Groschel D . Evaluation and diagnosis of acute infectious diarrhea. Am J Med. 1985; 78(6B):91-8. DOI: 10.1016/0002-9343(85)90370-5. View

Al-Hakim A, Hull R . Studies towards the development of chemically synthesized non-radioactive biotinylated nucleic acid hybridization probes. Nucleic Acids Res. 1986; 14(24):9965-76. PMC: 341348. DOI: 10.1093/nar/14.24.9965. View

10.

Boguslawski S, Smith D, Michalak M, Mickelson K, Yehle C, PATTERSON W . Characterization of monoclonal antibody to DNA.RNA and its application to immunodetection of hybrids. J Immunol Methods. 1986; 89(1):123-30. DOI: 10.1016/0022-1759(86)90040-2. View

11.

Totten P, Holmes K, Handsfield H, Knapp J, Perine P, Falkow S . DNA hybridization technique for the detection of Neisseria gonorrhoeae in men with urethritis. J Infect Dis. 1983; 148(3):462-71. DOI: 10.1093/infdis/148.3.462. View

12.

Ranki M, Palva A, Virtanen M, Laaksonen M, Soderlund H . Sandwich hybridization as a convenient method for the detection of nucleic acids in crude samples. Gene. 1983; 21(1-2):77-85. DOI: 10.1016/0378-1119(83)90149-x. View

13.

Blum H, Haase A, VYAS G . Molecular pathogenesis of hepatitis B virus infection: simultaneous detection of viral DNA and antigens in paraffin-embedded liver sections. Lancet. 1984; 2(8406):771-5. DOI: 10.1016/s0140-6736(84)90703-7. View

14.

Spector S, Rua J, Spector D, McMillan R . Detection of human cytomegalovirus in clinical specimens by DNA-DNA hybridization. J Infect Dis. 1984; 150(1):121-6. DOI: 10.1093/infdis/150.1.121. View

15.

Chan V, Fleming K, Mcgee J . Detection of sub-picogram quantities of specific DNA sequences on blot hybridization with biotinylated probes. Nucleic Acids Res. 1985; 13(22):8083-91. PMC: 322111. DOI: 10.1093/nar/13.22.8083. View

16.

Bradbury W, Pearson A, Marko M, Congi R, Penner J . Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis. J Clin Microbiol. 1984; 19(3):342-6. PMC: 271061. DOI: 10.1128/jcm.19.3.342-346.1984. View

17.

WALKER T, Rollinson D, Simpson A . Differentiation of Schistosoma haematobium from related species using cloned ribosomal RNA gene probes. Mol Biochem Parasitol. 1986; 20(2):123-31. DOI: 10.1016/0166-6851(86)90024-1. View

18.

Palva A, Ranki M . Microbial diagnosis by nucleic acid sandwich hybridization. Clin Lab Med. 1985; 5(3):475-90. View

19.

Wallace R, Shaffer J, Murphy R, Bonner J, Hirose T, Itakura K . Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch. Nucleic Acids Res. 1979; 6(11):3543-57. PMC: 327955. DOI: 10.1093/nar/6.11.3543. View

20.

Beckmann A, Myerson D, Daling J, Kiviat N, Fenoglio C, McDougall J . Detection and localization of human papillomavirus DNA in human genital condylomas by in situ hybridization with biotinylated probes. J Med Virol. 1985; 16(3):265-73. DOI: 10.1002/jmv.1890160307. View