The Application Analysis of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Pathogenic Bacterium in Peritoneal Dialysis-Associated Peritonitis

Overview

Journal Blood Purif

Specialty Hematology

Date 2019 Jan 3

PMID 30602148

Citations 1

Authors

Jing Yang

Xiang-Ming Qi

Yong-Gui Wu

Affiliations

Soon will be listed here.

Abstract

Background/aims: To estimate the clinical value of bacterial detection in peritoneal dialysis-associated peritonitis (PDAP) by multiplex real-time polymerase chain reaction (RT-PCR). This study was undertaken to evaluate multiplex RT-PCR for identifying clinically significant bacteria in PDAP.

Methods: Seventy peritoneal dialysate specimens were collected and traditional bacterial culture and universal primer RT-PCR detection of the bacterial were used.

Results: The positive rate of traditional culture method was 65.71% (46/70) and that of universal primer RT-PCR was 81.42% (57/70). For 6 clinical commonly pathogenic bacteria, multiplex, and monoplex RT-PCR all detected 38 positive ones within the 57 specimens that were detected positive by universal primer RT-PCR. The results of the 2 methods were completely identical. Detecting bacteria by universal primer PCR and Monoplex RT-PCR needs 4-5 and 6-9 h, respectively, while multiplex RT-PCR needs less than 3 h.

Conclusion: Our results demonstrated that the multiplex RT-PCR can detect several kinds of bacteria simultaneously and it is also more practical and convenient than monoplex RT-PCR.

Citing Articles

Cost-effective in-house COVID-19 reverse transcription-polymerase chain reaction testing with yeast-derived polymerase.

Zhra M, Al Saud A, Alzayer M, Okdah L, Tamim H, Fakhoury H Ann Thorac Med. 2024; 19(2):165-171.

PMID: 38766371 PMC: 11100475. DOI: 10.4103/atm.atm_180_23.

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