Detection and Genetic Characterization of Porcine Pegivirus from Pigs in China

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 2019 Jan 3

PMID 30600430

Citations 4

Authors

Dan Lei

Yu Ye

Kun Lin

Fanfan Zhang

Dongyan Huang

Kai Li

Weifeng Yuan

Qiong Wu

Zhen Ding

Leyi Wang

Deping Song

Yuxin Tang

Affiliations

Soon will be listed here.

Abstract

Porcine pegiviruses (PPgV) have been first discovered in serum samples from domestic pigs in Germany in 2016 and then in the USA in 2018. To date, there is no documentation with respect to the presence of PPgVs in domestic pigs in China. Herein, we attempted to determine the presence and prevalence of PPgV in China and its genetic characterization. In this study, 469 sera were tested and 34 (7.25%) were positive for PPgV. An ascending trend of the positive rate for PPgV was observed from suckling piglets (1.61%) to nursing piglets (1.85%), finishing pigs (6.56%), and sows (11.34%). The complete genome sequence of a representative strain of PPgV, PPgV_GDCH2017, and the complete E2 gene of 17 PPgV isolates discovered in this study was determined. Sequence analysis indicated that PPgV_GDCH2017 was highly related to other PPgVs with nucleotide and amino acid identities ranging from 87.3 to 97.4% and 94.6-99.3%, respectively, in the complete coding region. Phylogenetic analyses demonstrated that the PPgV_GDCH2017 discovered in this study was closely related to the PPgVs from the USA and clustered in the same genus with pegiviruses from other hosts. The topology of the phylogenetic tree based on the complete E2 gene was consistent with that based on the complete genome of PPgV. Further studies on pathogenicity and pathogenesis of PPgVs are needed.

Citing Articles

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The Prevalence, Genetic Characterization, and Evolutionary Analysis of Porcine Pegivirus in Guangdong, China.

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Genetic variability of porcine pegivirus in pigs from Europe and China and insights into tissue tropism.

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References

Koonin E . The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. J Gen Virol. 1991; 72 ( Pt 9):2197-206. DOI: 10.1099/0022-1317-72-9-2197. View

Epstein J, Quan P, Briese T, Street C, Jabado O, Conlan S . Identification of GBV-D, a novel GB-like flavivirus from old world frugivorous bats (Pteropus giganteus) in Bangladesh. PLoS Pathog. 2010; 6:e1000972. PMC: 2895649. DOI: 10.1371/journal.ppat.1000972. View

Stapleton J, Foung S, Muerhoff A, Bukh J, Simmonds P . The GB viruses: a review and proposed classification of GBV-A, GBV-C (HGV), and GBV-D in genus Pegivirus within the family Flaviviridae. J Gen Virol. 2010; 92(Pt 2):233-46. PMC: 3081076. DOI: 10.1099/vir.0.027490-0. View

Schwarze-Zander C, Blackard J, Rockstroh J . Role of GB virus C in modulating HIV disease. Expert Rev Anti Infect Ther. 2012; 10(5):563-72. PMC: 3499065. DOI: 10.1586/eri.12.37. View

Chandriani S, Skewes-Cox P, Zhong W, Ganem D, Divers T, Van Blaricum A . Identification of a previously undescribed divergent virus from the Flaviviridae family in an outbreak of equine serum hepatitis. Proc Natl Acad Sci U S A. 2013; 110(15):E1407-15. PMC: 3625295. DOI: 10.1073/pnas.1219217110. View

Kapoor A, Simmonds P, Scheel T, Hjelle B, Cullen J, Burbelo P . Identification of rodent homologs of hepatitis C virus and pegiviruses. mBio. 2013; 4(2):e00216-13. PMC: 3622934. DOI: 10.1128/mBio.00216-13. View

Kapoor A, Simmonds P, Cullen J, Scheel T, Medina J, Giannitti F . Identification of a pegivirus (GB virus-like virus) that infects horses. J Virol. 2013; 87(12):7185-90. PMC: 3676142. DOI: 10.1128/JVI.00324-13. View

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S . MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013; 30(12):2725-9. PMC: 3840312. DOI: 10.1093/molbev/mst197. View

Chang C, Stapleton J, Klinzman D, McLinden J, Purdue M, Katki H . GBV-C infection and risk of NHL among U.S. adults. Cancer Res. 2014; 74(19):5553-60. PMC: 4184918. DOI: 10.1158/0008-5472.CAN-14-0209. View

10.

Finn R, Coggill P, Eberhardt R, Eddy S, Mistry J, Mitchell A . The Pfam protein families database: towards a more sustainable future. Nucleic Acids Res. 2015; 44(D1):D279-85. PMC: 4702930. DOI: 10.1093/nar/gkv1344. View

11.

Baechlein C, Grundhoff A, Fischer N, Alawi M, Hoeltig D, Waldmann K . Pegivirus Infection in Domestic Pigs, Germany. Emerg Infect Dis. 2016; 22(7):1312-4. PMC: 4918184. DOI: 10.3201/eid2207.160024. View

12.

Singh S, Blackard J . Human pegivirus (HPgV) infection in sub-Saharan Africa-A call for a renewed research agenda. Rev Med Virol. 2017; 27(6). DOI: 10.1002/rmv.1951. View

13.

Yang C, Wang L, Shen H, Zheng Y, Bade S, Gauger P . Detection and genetic characterization of porcine pegivirus in pigs in the United States. Transbound Emerg Dis. 2018; 65(3):618-626. DOI: 10.1111/tbed.12844. View

14.

Simons J, Leary T, Dawson G, Desai S, Schlauder G, Muerhoff A . Identification of two flavivirus-like genomes in the GB hepatitis agent. Proc Natl Acad Sci U S A. 1995; 92(8):3401-5. PMC: 42174. DOI: 10.1073/pnas.92.8.3401. View

15.

Koonin E, Dolja V . Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol. 1993; 28(5):375-430. DOI: 10.3109/10409239309078440. View

16.

Leary T, Desai S, Erker J, MUSHAHWAR I . The sequence and genomic organization of a GB virus A variant isolated from captive tamarins. J Gen Virol. 1997; 78 ( Pt 9):2307-13. DOI: 10.1099/0022-1317-78-9-2307. View

17.

Adams N, Prescott L, Jarvis L, Lewis J, McClure M, Smith D . Detection in chimpanzees of a novel flavivirus related to GB virus-C/hepatitis G virus. J Gen Virol. 1998; 79 ( Pt 8):1871-7. DOI: 10.1099/0022-1317-79-8-1871. View