Lipid Peroxidation, Antioxidative Defense and Level of 8-hydroxy-2-deoxyguanosine in Cervical Cancer Patients

Overview

Journal J Med Biochem

Specialty Biochemistry

Date 2019 Jan 2

PMID 30598631

Citations 12

Authors

Marija Jelic

Aljosa Mandic

Nebojsa Kladar

Jan Sudji

Biljana Bozin

Branislava Srdjenovic

Affiliations

Soon will be listed here.

Abstract

Background: Oxidative stress has been associated with cervical cancer. Our aim was to examine lipid peroxidation and the extent of oxidative stress in women diagnosed with different stages of cervical cancer in order to evaluate its potential role in the evolution of cancer.

Methods: We measured the concentration of thiobarbituric acid reactive substances, activities of antioxidative enzymes and 8-hydroxy-2-deoxyguanosine in 153 subjects. Enzymatic activity as well as TBARS concentration were measured spectrophotometrically, while 8-OHdG was determined by gas chromatography-mass spectrometry. PPatients were categorized: group II H-SIL; group III FIGO Ia-Ib and group IV FIGO IIa-IV.

Results: Our results showed highly significant increase in the level of lipid peroxidation in group IV when com pared to the control group, group II and group III (p<0.001). Activity of superoxide dismutase was also significantly higher in group IV when compared to control group (p<0.01), group II (p<0.01) and group III (p<0.05). Activity of catalase was also significantly higher in group IV when compared to control group (p<0.005), group II (p<0.005) and group III (p<0.05). Activity of glutathione-S-transferase was also significantly higher in group IV when compared to control group (p<0.05), group II (p<0.05) and group III (p<0.05). Activities of glutathione peroxidase and glutathione reductase showed no significant differences among the groups. Level of 8-OHdG was significantly higher in group IV than in the other groups (p<0.01).

Conclusions: It can be concluded that oxidative stress is possibly involved in the pathogenesis of cervical cancer, demonstrated by increased lipid peroxidation and an altered antioxidant defense system and higher levels of 8-OHdG.

Citing Articles

The Potential of Hemp Extracts to Modify the Course of Oxidative-Stress Related Conditions.

Bijelic K, Srdjenovic conic B, Prpa B, Pilija V, Vukmirovic S, Kladar N Plants (Basel). 2024; 13(12).

PMID: 38931062 PMC: 11207586. DOI: 10.3390/plants13121630.

Crosstalk between ferroptosis and steroid hormone signaling in gynecologic cancers.

Lai W, Chen J, Wang T, Liu Q Front Mol Biosci. 2023; 10:1223493.

PMID: 37469703 PMC: 10352791. DOI: 10.3389/fmolb.2023.1223493.

A study on UHPLC-MS/MS analyses of DNA and RNA oxidative damage metabolites in patients with cervical carcinoma: 8-oxoG in urine as a potential biomarker of cervical carcinoma.

Lin R, Li X, Weng Q, Zhou X, Zheng F, Cai J Heliyon. 2022; 8(4):e09321.

PMID: 35520626 PMC: 9061785. DOI: 10.1016/j.heliyon.2022.e09321.

Effect of chocolate on older patients with cancer in palliative care: a randomised controlled study.

Vettori J, da-Silva L, Pfrimer K, Jordao A, Louzada-Junior P, Moriguti J BMC Palliat Care. 2022; 21(1):5.

PMID: 34980096 PMC: 8725414. DOI: 10.1186/s12904-021-00893-1.

The Oxidative Stress Parameters as Useful Tools in Evaluating the DNA Damage and Changes in the Complete Blood Count in Hospital Workers Exposed to Low Doses of Antineoplastic Drugs and Ionizing Radiation.

Mrdjanovic J, Solajic S, Srdenovic-conic B, Bogdanovic V, Dea K, Kladar N Int J Environ Res Public Health. 2021; 18(16).

PMID: 34444191 PMC: 8394042. DOI: 10.3390/ijerph18168445.

References

Chiou J, Hu M . Elevated lipid peroxidation and disturbed antioxidant enzyme activities in plasma and erythrocytes of patients with uterine cervicitis and myoma. Clin Biochem. 1999; 32(3):189-92. DOI: 10.1016/s0009-9120(98)00110-6. View

Romano G, Sgambato A, Mancini R, Capelli G, Giovagnoli M, Flamini G . 8-hydroxy-2'-deoxyguanosine in cervical cells: correlation with grade of dysplasia and human papillomavirus infection. Carcinogenesis. 2000; 21(6):1143-7. View

Chung-Man Ho J, Zheng S, Comhair S, Farver C, Erzurum S . Differential expression of manganese superoxide dismutase and catalase in lung cancer. Cancer Res. 2001; 61(23):8578-85. View

Hristozov D, Gadjeva V, Vlaykova T, Dimitrov G . Evaluation of oxidative stress in patients with cancer. Arch Physiol Biochem. 2002; 109(4):331-6. DOI: 10.1076/apab.109.4.331.4248. View

Kumaraguruparan R, Subapriya R, Viswanathan P, Nagini S . Tissue lipid peroxidation and antioxidant status in patients with adenocarcinoma of the breast. Clin Chim Acta. 2002; 325(1-2):165-70. DOI: 10.1016/s0009-8981(02)00292-9. View

Kolanjiappan K, Manoharan S, Kayalvizhi M . Measurement of erythrocyte lipids, lipid peroxidation, antioxidants and osmotic fragility in cervical cancer patients. Clin Chim Acta. 2002; 326(1-2):143-9. DOI: 10.1016/s0009-8981(02)00300-5. View

Manju V, Kalaivani Sailaja J, Nalini N . Circulating lipid peroxidation and antioxidant status in cervical cancer patients: a case-control study. Clin Biochem. 2002; 35(8):621-5. DOI: 10.1016/s0009-9120(02)00376-4. View

Sgambato A, Zannoni G, Faraglia B, Camerini A, Tarquini E, Spada D . Decreased expression of the CDK inhibitor p27Kip1 and increased oxidative DNA damage in the multistep process of cervical carcinogenesis. Gynecol Oncol. 2004; 92(3):776-83. DOI: 10.1016/j.ygyno.2003.12.008. View

Sharma R, Yang Y, Sharma A, Awasthi S, Awasthi Y . Antioxidant role of glutathione S-transferases: protection against oxidant toxicity and regulation of stress-mediated apoptosis. Antioxid Redox Signal. 2004; 6(2):289-300. DOI: 10.1089/152308604322899350. View

10.

Kim S, Kim J, Ko Y, Koo J, Chung H, Lee-Kim Y . Changes in lipid peroxidation and antioxidant trace elements in serum of women with cervical intraepithelial neoplasia and invasive cancer. Nutr Cancer. 2004; 47(2):126-30. DOI: 10.1207/s15327914nc4702_3. View

11.

Mila-Kierzenkowska C, Kedziora-Kornatowska K, Wozniak A, Drewa T, Wozniak B, Drewa S . The effect of brachytherapy on antioxidant status and lipid peroxidation in patients with cancer of the uterine cervix. Cell Mol Biol Lett. 2004; 9(3):511-8. View

12.

Manoharan S, Kolanjiappan K, Kayalvizhi M . Enhanced lipid peroxidation and impaired enzymic antioxidant activities in the erythrocytes of patients with cervical carcinoma. Cell Mol Biol Lett. 2005; 9(4A):699-707. View

13.

Lee G, Chung H, Lee K, Ahn H . Antioxidant vitamins and lipid peroxidation in patients with cervical intraepithelial neoplasia. J Korean Med Sci. 2005; 20(2):267-72. PMC: 2808604. DOI: 10.3346/jkms.2005.20.2.267. View

14.

Nakabeppu Y, Sakumi K, Sakamoto K, Tsuchimoto D, Tsuzuki T, Nakatsu Y . Mutagenesis and carcinogenesis caused by the oxidation of nucleic acids. Biol Chem. 2006; 387(4):373-9. DOI: 10.1515/BC.2006.050. View

15.

Beevi S, Rasheed M, Geetha A . Evidence of oxidative and nitrosative stress in patients with cervical squamous cell carcinoma. Clin Chim Acta. 2006; 375(1-2):119-23. DOI: 10.1016/j.cca.2006.06.028. View

16.

Valko M, Leibfritz D, Moncol J, Cronin M, Mazur M, Telser J . Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2006; 39(1):44-84. DOI: 10.1016/j.biocel.2006.07.001. View

17.

Sharma A, Rajappa M, Saxena A, Sharma M . Antioxidant status in advanced cervical cancer patients undergoing neoadjuvant chemoradiation. Br J Biomed Sci. 2007; 64(1):23-7. DOI: 10.1080/09674845.2007.11732751. View

18.

Wozniak B, Mila-Kierzenkowska C, Kedziora-Kornatowska K, Drewa T, Drewa G, Wozniak A . Influence of the management of cervical carcinoma on the activity of catalase and glutathione peroxidase in erythrocytes. Eur J Gynaecol Oncol. 2008; 28(6):461-3. View

19.

Looi M, Mohd Dali A, Md Ali S, Wan Ngah W, Mohd Yusof Y . Oxidative damage and antioxidant status in patients with cervical intraepithelial neoplasia and carcinoma of the cervix. Eur J Cancer Prev. 2008; 17(6):555-60. DOI: 10.1097/CEJ.0b013e328305a10b. View

20.

Srivastava S, Natu S, Gupta A, Pal K, Singh U, Agarwal G . Lipid peroxidation and antioxidants in different stages of cervical cancer: Prognostic significance. Indian J Cancer. 2009; 46(4):297-302. DOI: 10.4103/0019-509X.55549. View