Genotyping and Antibiotic Resistance Properties of Strains Isolated from Human and Animal Gastric Biopsies

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2018 Dec 28

PMID 30588039

Citations 14

Authors

Reza Ranjbar

Mohammad Chehelgerdi

Affiliations

Soon will be listed here.

Abstract

Purpose: The present study was done to assess the prevalence rate, antibiotic resistance pattern and genotyping status of the strains isolated from human and animal gastric biopsy samples.

Patients And Methods: A total of 1,150 gastric biopsy samples were randomly collected from humans (children and adults) and animals (cows, sheep and goats). All samples were subjected to culture, urease test and histopathologic examination. isolates were also confirmed using the gene PCR-amplification. Antibiotic resistance pattern was assessed by the disk diffusion method. Distribution of different genotypes was studied by PCR.

Results: The prevalence of in gastric biopsy samples which were studied using urease test, culture and histological examination were 57.04%, 55.40% and 60.80%, respectively. Samples that were collected from adult humans (78%) and sheep (70%) had the highest prevalence of strains, while those of goats (0.6%) and cows (4%) had the lowest. Findings of the culture method were confirmed using PCR-based amplification of . Distribution of among the gastric ulcers, duodenal ulcers, chronic gastritis gastric cancer and chronic cancer samples were 10.40%, 15.70%, 96.50%, 0.60% and 3.14%, respectively. strains harbored the highest prevalence of resistance against ampicillin (74.4%), clarithromycin (63.4%), trimethoprim (61.5%) and metronidazole (61.5%). The most commonly detected genotypes among the strains isolated from different types of biopsy samples were (84.79%), (55.95%), (49.84%), (48.58%), (47.02%) and (47.02%).

Conclusion: High prevalence of antibiotic resistance and virulent genotypes indicates an important public health issue. Similarities in antibiotic resistance and genotyping pattern of strains isolated from humans and animals may show their similar routes of infection.

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