Pre-pubertal Exposure to High Temperature Impairs Ovarian and Adrenal Gland Function in Female Rats

Overview

Journal J Vet Med Sci

Specialty Veterinary Medicine

Date 2018 Dec 28

PMID 30587674

Citations 6

Authors

Meihua Zheng

Kentaro Nagaoka

Gen Watanabe

Affiliations

Soon will be listed here.

Abstract

The influence of different levels of heat exposure on the functions of ovarian and adrenal gland were investigated in pre-puberty female rats. Three-week old female rats were treated with control (26°C) or three higher temperatures (38, 40 and 42°C) for 2hr/day. After 9 days of treatment, blood samples were collected for measurement of luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol-17β, corticosterone, cholesterol and triglyceride. Adrenal glands, ovaries and liver were collected for analyzing gene expressions. Body and liver weight were significantly low in the 42°C heating group. Circulating LH and triglyceride in the 42°C heating group were significantly lower, and estradiol-17β, corticosterone and cholesterol were significantly higher than those of the control group. The gene expression of 3β-HSD and P450c21 in the adrenal gland; 3β-HSD, receptors of LH, FSH and estrogen in the ovary were significantly low in heated rats. The liver gene expressions of caspase 3 and NK-κB were significantly high in 42°C heated rats, suggesting that the ability of liver metabolic function reduced in the 42°C heated rats. These results demonstrated that the high temperature is responsible for suppression of ovarian function by decreasing the expression of steroidogenic enzymes, estrogen and gonadotropin receptors in the ovary. Increase in circulating estradiol-17β in the heated rats may be due to accumulate this hormone in circulation by potential changes in liver metabolism during the heat stress.

Citing Articles

Allostasis in Neuroendocrine Systems Controlling Reproduction.

Carrasco R, Breen K Endocrinology. 2023; 164(10).

PMID: 37586095 PMC: 10461221. DOI: 10.1210/endocr/bqad125.

Climate change and pregnancy complications: From hormones to the immune response.

Yuzen D, Graf I, Diemert A, Arck P Front Endocrinol (Lausanne). 2023; 14:1149284.

PMID: 37091849 PMC: 10113645. DOI: 10.3389/fendo.2023.1149284.

Characteristics of menstrual cycle disorder and saliva metabolomics of young women in a high-temperature environment.

Wei M, An G, Fan L, Chen X, Li C, Chen J Front Physiol. 2023; 13:994990.

PMID: 36714308 PMC: 9880290. DOI: 10.3389/fphys.2022.994990.

Understanding how environmental factors influence reproductive aspects of wild myomorphic and hystricomorphic rodents.

Dantas M, Souza-Junior J, Castelo T, Lago A, Silva A Anim Reprod. 2021; 18(1):e20200213.

PMID: 33936293 PMC: 8078862. DOI: 10.1590/1984-3143-AR2020-0213.

Integrative Analysis of Vaginal Microorganisms and Serum Metabolomics in Rats With Estrous Cycle Disorder Induced by Long-Term Heat Exposure Based on 16S rDNA Gene Sequencing and LC/MS-Based Metabolomics.

An G, Zhang Y, Fan L, Chen J, Wei M, Li C Front Cell Infect Microbiol. 2021; 11:595716.

PMID: 33738264 PMC: 7962411. DOI: 10.3389/fcimb.2021.595716.

References

Al-Katanani Y, Webb D, Hansen P . Factors affecting seasonal variation in 90-day nonreturn rate to first service in lactating Holstein cows in a hot climate. J Dairy Sci. 2000; 82(12):2611-6. DOI: 10.3168/jds.S0022-0302(99)75516-5. View

Djordjevic J, Cvijic G, Davidovic V . Different activation of ACTH and corticosterone release in response to various stressors in rats. Physiol Res. 2003; 52(1):67-72. View

Gangwar P, BRANTON C, Evans D . REPRODUCTIVE AND PHYSIOLOGICAL RESPONSES OF HOLSTEIN HEIFERS TO CONTROLLED AND NATURAL CLIMATIC CONDITIONS. J Dairy Sci. 1965; 48:222-7. DOI: 10.3168/jds.s0022-0302(65)88200-5. View

Ray D, Halbach T, Armstrong D . Season and lactation number effects on milk production and reproduction of dairy cattle in Arizona. J Dairy Sci. 1992; 75(11):2976-83. DOI: 10.3168/jds.S0022-0302(92)78061-8. View

Bridges P, Brusie M, Fortune J . Elevated temperature (heat stress) in vitro reduces androstenedione and estradiol and increases progesterone secretion by follicular cells from bovine dominant follicles. Domest Anim Endocrinol. 2005; 29(3):508-22. DOI: 10.1016/j.domaniend.2005.02.017. View

Roman-Ponce H, Thatcher W, Wilcox C . Hormonal interelationships and physiological responses of lactating dairy cows to a shade management system in a subtropical environment. Theriogenology. 1981; 16(2):139-54. DOI: 10.1016/0093-691x(81)90097-2. View

Gwazdauskas F, Thatcher W, Kiddy C, Paape M, Wilcox C . Hormonal patterns during heat stress following PGF(2)alpha-tham salt induced luteal regression in heifers. Theriogenology. 1981; 16(3):271-85. DOI: 10.1016/0093-691x(81)90012-1. View

Thompson J, Magee D, Tomaszewski M, Wilks D, Fourdraine R . Management of summer infertility in Texas Holstein dairy cattle. Theriogenology. 1996; 46(3):547-58. DOI: 10.1016/0093-691x(96)00176-8. View

Webster Marketon J, Glaser R . Stress hormones and immune function. Cell Immunol. 2008; 252(1-2):16-26. DOI: 10.1016/j.cellimm.2007.09.006. View

10.

Schmittgen T, Livak K . Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008; 3(6):1101-8. DOI: 10.1038/nprot.2008.73. View

11.

Pariante C, Lightman S . The HPA axis in major depression: classical theories and new developments. Trends Neurosci. 2008; 31(9):464-8. DOI: 10.1016/j.tins.2008.06.006. View

12.

Meethal S, Liu T, Chan H, Ginsburg E, Wilson A, Gray D . Identification of a regulatory loop for the synthesis of neurosteroids: a steroidogenic acute regulatory protein-dependent mechanism involving hypothalamic-pituitary-gonadal axis receptors. J Neurochem. 2009; 110(3):1014-27. PMC: 2789665. DOI: 10.1111/j.1471-4159.2009.06192.x. View

13.

Hansen P . Effects of heat stress on mammalian reproduction. Philos Trans R Soc Lond B Biol Sci. 2009; 364(1534):3341-50. PMC: 2781849. DOI: 10.1098/rstb.2009.0131. View

14.

Yu J, Yin P, Liu F, Cheng G, Guo K, Lu A . Effect of heat stress on the porcine small intestine: a morphological and gene expression study. Comp Biochem Physiol A Mol Integr Physiol. 2010; 156(1):119-28. DOI: 10.1016/j.cbpa.2010.01.008. View

15.

Ren L, Li X, Weng Q, Trisomboon H, Yamamoto T, Pan L . Effects of acute restraint stress on sperm motility and secretion of pituitary, adrenocortical and gonadal hormones in adult male rats. J Vet Med Sci. 2010; 72(11):1501-6. DOI: 10.1292/jvms.10-0113. View

16.

Taya K, Sasamoto S . Inhibitory effects of corticotrophin-releasing factor and beta-endorphin on LH and FSH secretion in the lactating rat. J Endocrinol. 1989; 120(3):509-15. DOI: 10.1677/joe.0.1200509. View

17.

Clarke I, Bartolini D, Conductier G, Henry B . Stress Increases Gonadotropin Inhibitory Hormone Cell Activity and Input to GnRH Cells in Ewes. Endocrinology. 2016; 157(11):4339-4350. DOI: 10.1210/en.2016-1513. View

18.

Oyola M, Handa R . Hypothalamic-pituitary-adrenal and hypothalamic-pituitary-gonadal axes: sex differences in regulation of stress responsivity. Stress. 2017; 20(5):476-494. PMC: 5815295. DOI: 10.1080/10253890.2017.1369523. View

19.

Joseph D, Whirledge S . Stress and the HPA Axis: Balancing Homeostasis and Fertility. Int J Mol Sci. 2017; 18(10). PMC: 5666903. DOI: 10.3390/ijms18102224. View

20.

Iwasa T, Matsuzaki T, Yano K, Mayila Y, Irahara M . The roles of kisspeptin and gonadotropin inhibitory hormone in stress-induced reproductive disorders. Endocr J. 2018; 65(2):133-140. DOI: 10.1507/endocrj.EJ18-0026. View