70-kD Heat Shock-related Protein is One of at Least Two Distinct Cytosolic Factors Stimulating Protein Import into Mitochondria

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1988 Dec 1

PMID 3058716

Citations 116

Authors

H Murakami

D Pain

G Blobel

Affiliations

Soon will be listed here.

Abstract

We have developed an in vitro system in which the posttranslational import of Put2 (delta-pyrroline-5-carboxylate dehydrogenase), into yeast mitochondria is dependent on the addition of yeast postribosomal supernatant (PRS). When mRNA for a nuclear-encoded yeast mitochondrial matrix protein, Put2, was translated in a wheat germ cell-free system, import into posttranslationally added yeast mitochondria was negligible. However, when a yeast PRS was added, significant import was observed. The import stimulating activity of the yeast PRS was shown to consist of at least two distinct factors. One of these is the recently purified 70-kD heat shock-related protein Ssalp/Ssa2p, two proteins that are 98% homologous. The other factor is an N-ethylmaleimide-sensitive protein(s). Both factors act synergistically.

Citing Articles

Heat shock protein 70 gene polymorphisms in Iranian patients with Multiple sclerosis.

Bakhshandeh A, Dolatabadi A, Farazmanfar T, Shahbazi M Am J Clin Exp Immunol. 2025; 13(6):278-284.

PMID: 39839348 PMC: 11744348. DOI: 10.62347/CMYA9839.

Interactions of amyloidogenic proteins with mitochondrial protein import machinery in aging-related neurodegenerative diseases.

Reed A, Mitchell W, Alexandrescu A, Alder N Front Physiol. 2023; 14:1263420.

PMID: 38028797 PMC: 10652799. DOI: 10.3389/fphys.2023.1263420.

Genetic suppressors of Δgrx3 Δgrx4, lacking redundant multidomain monothiol yeast glutaredoxins, rescue growth and iron homeostasis.

Li G, Nanjaraj Urs A, Dancis A, Zhang Y Biosci Rep. 2022; 42(6).

PMID: 35593209 PMC: 9202360. DOI: 10.1042/BSR20212665.

Sensing, signaling and surviving mitochondrial stress.

Eckl E, Ziegemann O, Krumwiede L, Fessler E, Jae L Cell Mol Life Sci. 2021; 78(16):5925-5951.

PMID: 34228161 PMC: 8316193. DOI: 10.1007/s00018-021-03887-7.

Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis.

Niemi N, Wilson G, Overmyer K, Vogtle F, Myketin L, Lohman D Nat Commun. 2019; 10(1):3197.

PMID: 31324765 PMC: 6642090. DOI: 10.1038/s41467-019-11047-6.

References

Maccecchini M, Rudin Y, Blobel G, Schatz G . Import of proteins into mitochondria: precursor forms of the extramitochondrially made F1-ATPase subunits in yeast. Proc Natl Acad Sci U S A. 1979; 76(1):343-7. PMC: 382935. DOI: 10.1073/pnas.76.1.343. View

Chirico W, Waters M, Blobel G . 70K heat shock related proteins stimulate protein translocation into microsomes. Nature. 1988; 332(6167):805-10. DOI: 10.1038/332805a0. View

McAda P, DOUGLAS M . A neutral metallo endoprotease involved in the processing of an F1-ATPase subunit precursor in mitochondria. J Biol Chem. 1982; 257(6):3177-82. View

Argan C, LUSTY C, Shore G . Membrane and cytosolic components affecting transport of the precursor for ornithine carbamyltransferase into mitochondria. J Biol Chem. 1983; 258(11):6667-70. View

Miura S, Mori M, TATIBANA M . Transport of ornithine carbamoyltransferase precursor into mitochondria. Stimulation by potassium ion, magnesium ion, and a reticulocyte cytosolic protein(s). J Biol Chem. 1983; 258(11):6671-4. View

Brandriss M . Proline utilization in Saccharomyces cerevisiae: analysis of the cloned PUT2 gene. Mol Cell Biol. 1983; 3(10):1846-56. PMC: 370046. DOI: 10.1128/mcb.3.10.1846-1856.1983. View

Erickson A, Blobel G . Cell-free translation of messenger RNA in a wheat germ system. Methods Enzymol. 1983; 96:38-50. DOI: 10.1016/s0076-6879(83)96007-x. View

Gasser S . Import of polypeptides into isolated yeast mitochondria. Methods Enzymol. 1983; 97:329-36. DOI: 10.1016/0076-6879(83)97145-8. View

Ohta S, Schatz G . A purified precursor polypeptide requires a cytosolic protein fraction for import into mitochondria. EMBO J. 1984; 3(3):651-7. PMC: 557402. DOI: 10.1002/j.1460-2075.1984.tb01862.x. View

10.

Craig E, Jacobsen K . Mutations of the heat inducible 70 kilodalton genes of yeast confer temperature sensitive growth. Cell. 1984; 38(3):841-9. DOI: 10.1016/0092-8674(84)90279-4. View

11.

Firgaira F, Hendrick J, KALOUSEK F, Kraus J, ROSENBERG L . RNA required for import of precursor proteins into mitochondria. Science. 1984; 226(4680):1319-22. DOI: 10.1126/science.6209799. View

12.

Krzywicki K, Brandriss M . Primary structure of the nuclear PUT2 gene involved in the mitochondrial pathway for proline utilization in Saccharomyces cerevisiae. Mol Cell Biol. 1984; 4(12):2837-42. PMC: 369295. DOI: 10.1128/mcb.4.12.2837-2842.1984. View

13.

Argan C, Shore G . The precursor to ornithine carbamyl transferase is transported to mitochondria as a 5S complex containing an import factor. Biochem Biophys Res Commun. 1985; 131(1):289-98. DOI: 10.1016/0006-291x(85)91801-7. View

14.

Waters M, Blobel G . Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis. J Cell Biol. 1986; 102(5):1543-50. PMC: 2114218. DOI: 10.1083/jcb.102.5.1543. View

15.

Pelham H . Speculations on the functions of the major heat shock and glucose-regulated proteins. Cell. 1986; 46(7):959-61. DOI: 10.1016/0092-8674(86)90693-8. View

16.

Waters M, Chirico W, Blobel G . Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor. J Cell Biol. 1986; 103(6 Pt 2):2629-36. PMC: 2114630. DOI: 10.1083/jcb.103.6.2629. View

17.

Pain D, Blobel G . Protein import into chloroplasts requires a chloroplast ATPase. Proc Natl Acad Sci U S A. 1987; 84(10):3288-92. PMC: 304854. DOI: 10.1073/pnas.84.10.3288. View

18.

Pfanner N, Neupert W . Distinct steps in the import of ADP/ATP carrier into mitochondria. J Biol Chem. 1987; 262(16):7528-36. View

19.

Fecycz I, Blobel G . Soluble factors stimulating secretory protein translocation in bacteria and yeast can substitute for each other. Proc Natl Acad Sci U S A. 1987; 84(11):3723-7. PMC: 304948. DOI: 10.1073/pnas.84.11.3723. View

20.

Werner-Washburne M, Stone D, Craig E . Complex interactions among members of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae. Mol Cell Biol. 1987; 7(7):2568-77. PMC: 365392. DOI: 10.1128/mcb.7.7.2568-2577.1987. View